The Protein Journal

, Volume 28, Issue 7–8, pp 333–340 | Cite as

Characterization and Genetic Study of the Ovine α S2 -Casein (CSN1S2) Allele B

  • G. Picariello
  • D. Rignanese
  • S. Chessa
  • G. Ceriotti
  • A. Trani
  • A. Caroli
  • A. Di Luccia


An interesting and quite complex protein pattern has been described at ovine milk proteins but the genetic control of the variation observed was assessed only in few cases. The aim of this work was to characterize the ovine α s2 -casein (CSN1S2) B variant, first observed in the Italian Gentile di Puglia, a fine-wooled ovine breed, and to investigate its occurrence in two further breeds, the Sarda and Camosciata, which are the most widespread dairy breeds in Italy. The B variant differs from the most common form A with two amino acid exchanges: Asp75 → Tyr75 and Ile105 → Val105. The first substitution, resulting in a loss of a negative charge, is responsible for the higher isoelectric point of the B protein variant, which allows its detection by isoelectric focusing electrophoresis (IEF). The occurrence of CSN1S2*B in Sarda and Comisana was demonstrated. Since the Asp75 → Tyr75 substitution modifies the protein electric charge, milk properties may result affected to some extent.


αs2-Casein Sheep Protein variant Mass spectrometry 





Delay extraction




High performance liquid chromatography




Liquid chromatography electrospray ionisation mass spectrometry


Mass spectrometry


Matrix assisted laser desorption ionization-time of flight


Isoelectric point


Post-source decay


Reversed phase


Trifluoroacetic acid


α-Cyano-4-hydroxycinnamic Acid



The research was supported by PRIN2005—2005075887_001.


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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • G. Picariello
    • 1
  • D. Rignanese
    • 2
  • S. Chessa
    • 3
  • G. Ceriotti
    • 3
  • A. Trani
    • 4
  • A. Caroli
    • 2
  • A. Di Luccia
    • 4
  1. 1.Istituto di Scienze dell’Alimentazione-Consiglio Nazionale delle RicercheAvellinoItaly
  2. 2.Dipartimento di Scienze Biomediche e BiotecnologieUniversità degli Studi di BresciaBresciaItaly
  3. 3.Dipartimento di Scienze e Tecnologie Veterinarie per la Sicurezza AlimentareUniversità degli Studi di MilanoMilanItaly
  4. 4.Dipartimento di Progettazione e Gestione dei Sistemi Agro-Zootecnici ForestaliUniversità degli Studi di BariBariItaly

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