Skip to main content
SpringerLink
Log in

Characterization and Genetic Study of the Ovine α S2 -Casein (CSN1S2) Allele B

  • Published:
The Protein Journal Aims and scope Submit manuscript

Abstract

An interesting and quite complex protein pattern has been described at ovine milk proteins but the genetic control of the variation observed was assessed only in few cases. The aim of this work was to characterize the ovine α s2 -casein (CSN1S2) B variant, first observed in the Italian Gentile di Puglia, a fine-wooled ovine breed, and to investigate its occurrence in two further breeds, the Sarda and Camosciata, which are the most widespread dairy breeds in Italy. The B variant differs from the most common form A with two amino acid exchanges: Asp75 → Tyr75 and Ile105 → Val105. The first substitution, resulting in a loss of a negative charge, is responsible for the higher isoelectric point of the B protein variant, which allows its detection by isoelectric focusing electrophoresis (IEF). The occurrence of CSN1S2*B in Sarda and Comisana was demonstrated. Since the Asp75 → Tyr75 substitution modifies the protein electric charge, milk properties may result affected to some extent.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

Abbreviations

CN:

Casein

DE:

Delay extraction

DTT:

Dithiothreitol

HPLC:

High performance liquid chromatography

IEF:

Isoelectrofocusing

LC-ESI/MS:

Liquid chromatography electrospray ionisation mass spectrometry

MS:

Mass spectrometry

MALDI-TOF:

Matrix assisted laser desorption ionization-time of flight

pI:

Isoelectric point

PSD:

Post-source decay

RP:

Reversed phase

TFA:

Trifluoroacetic acid

4-CHCA:

α-Cyano-4-hydroxycinnamic Acid

References

  1. Boisnard M, Petrissant G (1985) Biochimie 67:1043–1051

    Article  CAS  Google Scholar 

  2. Boisnard M, Hue D, Bouniol C, Mercier JC, Gaye P (1991) Eur J Biochem 201:633–641

    Article  CAS  Google Scholar 

  3. Bouniol C (1993) Gene 125:235–236

    Article  CAS  Google Scholar 

  4. Ceriotti G, Chessa S, Bolla P, Budelli E, Bianchi L, Duranti E, Caroli A (2004) J Dairy Sci 87:2606–2613

    Article  CAS  Google Scholar 

  5. Chessa S, Bolla P, Dario C, Pieragostini E, Caroli A (2003) Sci Tecn Latt-Cas 54:191–198

    Google Scholar 

  6. Chessa S, Budelli E, Caroli A, Pieragostini E (2003) Sci Tecn Latt-Cas 54:35–43

    CAS  Google Scholar 

  7. Chianese L, Garro G, Addeo F, López-Gálvez G, Ramos M (1993) J Dairy Res 60:485–493

    Article  CAS  Google Scholar 

  8. Erhardt G, Juszczak J, Panicke L, Krick-saleck H (1998) J Anim Breed Genet 115:63–71

    CAS  Google Scholar 

  9. Ferranti P, Chianese L, Malorni A, Migliaccio F, Stingo V, Addeo F (1998) J Agr Food Chem 46:411–416

    Article  CAS  Google Scholar 

  10. Ferranti P, Pizzano R, Garro G, Caira S, Chianese L, Addeo F (2001) J Dairy Res 68:35–51

    Article  CAS  Google Scholar 

  11. Jakob E, Puhan Z (1992) Int Dairy J 2:157–178

    Article  CAS  Google Scholar 

  12. Martin P, Szymanowska M, Zwierzchowski L, Leroux C (2002) Reprod Nutr Dev 42:433–459

    Article  CAS  Google Scholar 

  13. Moioli B, D’Andrea M, Pilla F (2007) Small Rumin Res 68:179–192

    Article  Google Scholar 

  14. Recio I, Ramos M, Amigo L (1997) J Dairy Res 64:525–534

    Article  CAS  Google Scholar 

Download references

Acknowledgment

The research was supported by PRIN2005—2005075887_001.

Author information

Authors and Affiliations

  1. Istituto di Scienze dell’Alimentazione-Consiglio Nazionale delle Ricerche, 83100, Avellino, Italy

    G. Picariello

  2. Dipartimento di Scienze Biomediche e Biotecnologie, Università degli Studi di Brescia, 25123, Brescia, Italy

    D. Rignanese & A. Caroli

  3. Dipartimento di Scienze e Tecnologie Veterinarie per la Sicurezza Alimentare, Università degli Studi di Milano, 20134, Milan, Italy

    S. Chessa & G. Ceriotti

  4. Dipartimento di Progettazione e Gestione dei Sistemi Agro-Zootecnici Forestali, Università degli Studi di Bari, 70126, Bari, Italy

    A. Trani & A. Di Luccia

Authors
  1. G. Picariello
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. Rignanese
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Chessa
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. Ceriotti
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. Trani
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. Caroli
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. A. Di Luccia
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. Di Luccia.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Picariello, G., Rignanese, D., Chessa, S. et al. Characterization and Genetic Study of the Ovine α S2 -Casein (CSN1S2) Allele B . Protein J 28, 333–340 (2009). https://doi.org/10.1007/s10930-009-9198-8

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10930-009-9198-8

Keywords

Search

Navigation