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Comparative Response of Indigenously Developed Bacterial Consortia on Progressive Degradation of Polyhydroxybutyrate Film Composites

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Abstract

The global demand of bioplastics has lead to an exponential increase in their production commercially. Hence, biodegradable nature needs to be evaluated in various ecosystems viz. air, water, soil and other environmental conditions to avoid the polymeric waste accumulation in the nature. In this paper, we investigated the progressive response of two indigenously developed bacterial consortia, i.e., consortium-I (C-I: Pseudomonas sp. strain Rb10, Pseudomonas sp. strain Rb11 and Bacillus sp. strain Rb18), and consortium-II (C-II: Lysinibacillus sp. strain Rb1, Pseudomonas sp. strain Rb13 and Pseudomonas sp. strain Rb19), against biodegradation behavior of polyhydroxybutyrate (PHB) film composites, under natural soil ecosystem (in net house). The biodegraded films recovered after 6 and 9 months of incubation were analyzed through Fourier transform infrared spectroscopy and scanning electron microscopy to determine the variations in chemical and morphological parameters (before and after incubation). Noticeable changes in the bond intensity, surface morphology and conductivity were found when PHB composites were treated with C-II. These changes were drastic in case of blends in comparison to copolymer. The potential isolates not only survived, but, also, there was a significant increase in bacterial diversity during whole period of incubation. To the best of our knowledge, it is the first report which described the biodegradation potential of Lysinibacillus sp. as a part of C-II with Pseudomonas sp. against PHB film composites.

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Acknowledgements

Senior author (SR) acknowledges “Department of Science and Technology” for INSPIRE Junior Research Fellowship for providing financial assistance during the course of this study. We thank Central Drug Research Institute, Lucknow, College of Veterinary and Animal Sciences, Pantnagar, and Fraunhofer Institute IPK, Berlin, Germany for FT-IR, SEM analysis, and PHB film specimens, respectively.

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Authors and Affiliations

  1. Department of Microbiology, C.B.S.H., G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, 263145, India

    Shikha Raghuwanshi, Saurabh Kumar & Reeta Goel

  2. Department of Chemistry, C.B.S.H., G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, 263145, India

    M. G. H. Zaidi

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  1. Shikha Raghuwanshi
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Correspondence to Shikha Raghuwanshi.

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Raghuwanshi, S., Zaidi, M.G.H., Kumar, S. et al. Comparative Response of Indigenously Developed Bacterial Consortia on Progressive Degradation of Polyhydroxybutyrate Film Composites. J Polym Environ 26, 2661–2675 (2018). https://doi.org/10.1007/s10924-017-1159-2

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