Tailored Natural Polysaccharides Beads as Green Sorbents for Efficient Lysozyme Adsorption
- 99 Downloads
Herein, we prepared alginate (SA)/carboxymethyl cellulose (CMC) gel beads through Ca2+ and glutaraldehyde (GA) crosslinking and investigated their adsorption performance on lysozyme from aqueous solution. Taking advantage of the abundant active carboxyl groups on SA and CMC, the obtained SA/CMC gel beads present an excellent integrated lysozyme adsorption performance with a high capacity of 236.34 mg g−1, short equilibrium time of 8 h, ease of elution, and good reusability. Furthermore, the resultant SA/CMC gel beads also possess unique selectivity for positively charged proteins, confirmed by the method of sodium dodecyl sulfate polyacrylamide gel electrophoresis. Considering the nature of biopolymers and advantages of favorable physical properties, high efficiency, cost-effectiveness, intriguing adsorption capacity and easier separation from the reaction system, the SA/CMC gel beads may find more potential in protein separation and purification than that of synthetic material.
KeywordsAlginate Carboxymethyl cellulose Lysozyme Efficient adsorption
This work was partially supported by the China Scholarship Council (201506120259) and Weihai Science and Technology Development Plan Project (2013GNS028).
- 2.Kubo T, Arimura S, Tominaga Y, Naito T, Hosoya K, Otsuka K (2015) Molecularly imprinted polymers for selective adsorption of lysozyme and cytochromec using a PEG-based hydrogel: selective recognition for different conformations due to pH conditions. Macromolecules 48(12):4081–4087CrossRefGoogle Scholar
- 15.Dumitriu RP, Stoica I, Vasilescu DS, Cazacu G, Vasile C (2017) Alginate/lignosulfonate blends with photoprotective and antioxidant properties for active packaging applications. J Polym Environ. https://doi.org/10.1007/s10924-017-1018-1
- 18.Agnihotri S, Singhal R (2017) Synthesis and characterization of novel poly(acrylic acid/sodium alginate/sodium humate) superabsorbent hydrogels. Part II: the effect of SH variation on Cu2+, Pb2+, Fe2+ metal ions, MB, CV dye adsorption study. J Polym Environ. https://doi.org/10.1007/s10924-017-0956-y
- 32.Mir S, Yasin T, Siddiqi H, M, Murtaza G (2016) Thermal, rheological, mechanical and morphological behavior of high density polyethylene and carboxymethyl cellulose blend. J Polym Environ 25(4):1011–1020Google Scholar
- 39.Luo X, Lei X, Cai N, Xie X, Xue Y, Yu F (2016) Removal of heavy metal ions from water by magnetic cellulose-based beads with embedded chemically modified magnetite nanoparticles and activated carbon. ACS Sustainable Chem Eng 4(7):3960–3969Google Scholar
- 42.He X, Male KB, Nesterenko PN, Brabazon D, Paull B, Luong JH (2013) Adsorption and desorption of methylene blue on porous carbon monoliths and nanocrystalline cellulose. ACS Sustain Chem Eng 5(17):8796–8804Google Scholar