Skip to main content
SpringerLink
Log in

Production of 3-Hydroxybutyrate Monomers by Pseudomonas mendocina DS04-T Biodegraded Polyhydroxybutyrate

  • Original Paper
  • Published:
Journal of Polymers and the Environment Aims and scope Submit manuscript

Abstract

The production conditions of 3-hydroxybutyrate (3-HB) monomers in submerged culture using Pseudomonas mendocina DS04-T as a degrading strain were optimized. The optimal culture medium constituents (w/v) were determined as follows: 0.5 % PHB, 0.15 % NH4Cl, 1.2 % Na2HPO4·12H2O, 0.3 % KH2PO4, 0.05 % MgSO4·7H2O, and 0.0005 % CaCl2·2H2O. The optimum parameters for liquid fermentation were as follows: temperature, 30 °C; inoculum content, 1.0 %; cultivation time, 18 h; initial pH, 7.0; volume of medium, 100 mL; and rotary speed, 180 rpm. Yield of 3-HB monomer and PHB depolymerase activity at optimized conditions were (56.4 ± 0.8) % and (57.4 ± 2.6) U/mL, respectively. The 3-HB monomer concentration obtained under optimized conditions was 1.5 times that obtained under the basic culture medium and initial conditions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Lenz RW, Marchessault RH (2005) Biomacromolecules 6:1–8

    Article  CAS  Google Scholar 

  2. Hazer B, Steinbüchel A (2007) Appl Microbiol Biotechnol 74:1–12

    Article  CAS  Google Scholar 

  3. Ueda H, Tabata Y (2003) Adv Drug Deliv Rev 55:501–518

    Article  CAS  Google Scholar 

  4. Chen GQ (2009) Chem Soc Rev 38:2434–2446

    Article  CAS  Google Scholar 

  5. Cousley RR (2009) J Clin Orthod 43:403–407

    Google Scholar 

  6. Xu XY, Li XT, Peng SW, Xiao JF, Liu C, Fang G, Chen KC, Chen GQ (2010) Biomaterials 31:3967–3975

    Article  CAS  Google Scholar 

  7. Tokiwa Y, Calabia BP (2004) Biotechnol Lett 26:1181–1189

    Article  CAS  Google Scholar 

  8. Kim DY, Kim HW, Chung MG, Rhee YH (2007) J Microbiol 45:87–97

    Google Scholar 

  9. Luckachan GE, Pillai CKS (2011) J Polym Environ 19:637–676

    Article  CAS  Google Scholar 

  10. Jendrossek D, Handrick R (2002) Annu Rev Microbiol 56:403–432

    Article  CAS  Google Scholar 

  11. Chen GQ, Wu Q (2005) Appl Microbiol Biotechnol 67:592–599

    Article  CAS  Google Scholar 

  12. de Roo G, Kellerhals MB, Ren Q, Witholt B, Kessler B (2002) Biotechnol Bioeng 77:717–722

    Article  Google Scholar 

  13. Noyori R, Kitamura M, Ohkuma T (2004) Proc Natl Acad Sci USA 101:5356–5362

    Article  CAS  Google Scholar 

  14. Wang Z, Zhao C, Pierce ME, Fortunak JM (1999) Tetrahedron Asymmetry 10:225–228

    Article  CAS  Google Scholar 

  15. Nakahata M, Imaida M, Ozaki H, Harada T, Tai A (1982) Bull Chem Soc Jpn 55:2186–2189

    Article  CAS  Google Scholar 

  16. Brown HC, Ramachandran PV (1991) Pure Appl Chem 63:307–316

    Article  CAS  Google Scholar 

  17. Ikunaka M (2003) Chem Eur J 9:379–388

    Article  Google Scholar 

  18. Lee SY, Lee Y, Wang FL (1999) Biotechnol Bioeng 65:363–368

    Article  CAS  Google Scholar 

  19. Wang ZY, Wang Y, Guo ZQ, Li F, Chen S (2011) Polym Eng Sci 51:454–459

    Article  CAS  Google Scholar 

  20. Zhou HL, Wang ZY, Chen S, Liu DB, Xia HM (2009) Polym-Plast Technol 48:58–63

    Article  CAS  Google Scholar 

  21. Gao D, Maehara A, Yamane T, Ueda S (2001) FEMS Microbiol Lett 196:159–164

    Article  CAS  Google Scholar 

  22. Shirakura Y, Fukui T, Saito T, Okamoto Y, Narikawa T, Koide K, Tomita K, Takemasa T, Masamune S (1986) Biochem Biophys Acta 880:46–53

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by National Natural Science Foundation of China (Grant No. 31100099), Science Project of Liaoning Province Education Office (L2011060) and Science Foundation of Liaoning Shihua University (No. 2011XJJ-025).

Author information

Authors and Affiliations

  1. School of Environmental and Biological Engineering, Liaoning Shihua University, 113001, Fushun, People’s Republic of China

    Lin-lin Li, Jia Gao, Hu-sheng Jiang & Zhan-yong Wang

Authors
  1. Lin-lin Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jia Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hu-sheng Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zhan-yong Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhan-yong Wang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Li, Ll., Gao, J., Jiang, Hs. et al. Production of 3-Hydroxybutyrate Monomers by Pseudomonas mendocina DS04-T Biodegraded Polyhydroxybutyrate. J Polym Environ 21, 826–832 (2013). https://doi.org/10.1007/s10924-012-0553-z

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10924-012-0553-z

Keywords

Search

Navigation