A Simple Assay for Monitoring Cellulose in Paper-Spiked Soil
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We describe a simple method for measuring cellulose in soil. We used this method to measure the recovery of shredded office paper and pure medium fiber cellulose added to a Helvetia silt loam clay soil. This method consists of solvating cellulose from soil with 77% H2SO4 and analysis of the cellulose recovered by the phenol-sulfuric acid carbohydrate assay. Unlike previous related methods the modifications we propose allow good recovery of cellulose (~99%) and eliminate the need to autoclave the samples. We verified this method on soil spiked with up to 12% cellulose and found a good linear relationship between the amounts of cellulose added relative to that recovered. With proper fragmentation and dilution of the acid-treated soil samples, higher concentrations can be easily measured. We propose this technique as a robust and high throughput means to monitor the degradation of cellulose in paper spiked soil.
KeywordsCellulose Soil Method Phenol-sulfuric acid
We thank Portland State University and Bokashicycle LLC for support.
- 1.Walchli O (1978) Material und Organismen 13:101Google Scholar
- 5.Dennis VM, Winfield BA (1977) Compost Sci 18:27Google Scholar
- 18.Tinsley J (1950) In: Hoitsemo Brothers (eds) Trans. 4th Int. Congr. Soil Sci. Determination of organic carbon in soils by dichromate mixtures, Groningen, The Netherlands, vol 1, pp 161–169Google Scholar
- 20.Nelson DW, Sommers LE (1996) In: Sparks DL (ed) Total carbon organic carbon and organic matter. Methods of soil analysis Part 3, Chap. 34. Chemical Methods Soil Science Society of America and American Society of AgronomyGoogle Scholar