Journal of Polymers and the Environment

, Volume 14, Issue 3, pp 231–237 | Cite as

Effect of Cross-Linking Waste Protein with Diepoxides on its Biodegradation under Anaerobic Conditions

  • Jiří Pšeja
  • Josef Hrnčiřík
  • Jan Kupec
  • Hana Charvátová
  • Petr Hruzík
  • Michael Tupý


Biodegradability testing was performed in an aqueous environment under anaerobic conditions after inoculation with digested sludge from municipal wastewater treatment plant. In cross-linking with 1,2:3,4-diepoxybutane in limits 0.8–9.1% weight, biodegradability degree decreased from 76.8 to 62.2%; when 1,2:7,8 diepoxyoctane in quantities 1.1–13.2% weight was used, biodegradability degree dropped more prominently – from 72.3 to 22.8%. There is obviously a direct connection between growing cross-link degree (assessed by so-called fixation index) and decreasing readiness to biodegradation, apparently owing to build-up of a network forming an obstacle to access of micro-organisms and enzymes.


Biodegradation Anaerobic Cross-linking Proteins Hydrolyzate Diepoxides 



This work was accomplished with financial support for research by Research Projects of the Ministry of Youth, Education and Sports of the Czech Republic, No. 7088352101.


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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Jiří Pšeja
    • 1
  • Josef Hrnčiřík
    • 2
  • Jan Kupec
    • 1
  • Hana Charvátová
    • 3
  • Petr Hruzík
    • 1
  • Michael Tupý
    • 1
  1. 1.Department of Environment Protection Engineering, Faculty of TechnologyTomas Bata University in Zlín, námZlínCzech Republic
  2. 2.Department of Food Engineering and Chemistry, Faculty of TechnologyTomas Bata University in ZlínZlínCzech Republic
  3. 3.Faculty of Applied InformaticsInstitute of Process Control and Applied InformaticsZlínCzech Republic

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