Journal of Mammary Gland Biology and Neoplasia

, Volume 14, Issue 3, pp 261–268 | Cite as

Metabolic Adaptations During Lactogenesis

Fatty acid and lactose synthesis in cow mammary tissue
  • R. W. Mellenberger
  • D. E. Bauman
  • D. R. Nelson

1. Mammary-tissue biopsies were obtained from multiparous cows at 30 and 7 days pre partum and 7 and 40 days postpartum. Investigations of the effect of lactogenesis on fatty acid and lactose synthesis involved measurements of biosynthetic capacity (tissue-slice incubations in vitro) and activities ofrelevant enzymes. 2. Fatty acid synthesis from acetate increased over 20-fold from 30 days pre partum to 40 days post partum. Changes in the lipogenic capacity of mammary-tissue slices more closely paralleled increases in the activities of acetyl-CoA carboxylase (EC and acetyl-CoA synthetase (EC than of other enzymes involved in acetate incorporation into fatty acids or in NADPH generation. 3. Lactose biosynthesis by mammary-tissue slices, lactose synthetase activity (EC and α-lactalbumin concentration were all negligible at 30 days pre partum but increased 2.5-4-fold between 7 days pre partum and 40 days post partum. Phosphoglucomutase (EC, UDP-glucose pyrophosphorylase (EC and UDPglucose 4-epimerase (EC had substantial activities at 30 days pre partum and increased less dramatically during lactogenesis. 4. Results are consistent with acetyl-CoA carboxylase and perhaps acetyl-CoA synthetase representing the regulatory enzyme(s) in fatty acid synthesis, with lactose synthetase (α-lactalbumin) serving a similar function in lactose biosynthesis.


Lactose Fatty Acid Synthesis Post Partum Phosphoglucomutase Acetate Incorporation 
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Copyright information

© Dale E Bauman et al 2009

Authors and Affiliations

  • R. W. Mellenberger
    • 1
    • 2
  • D. E. Bauman
    • 1
  • D. R. Nelson
    • 3
  1. 1.Department ofDairy ScienceUniversity ofIllinoisUrbanaUSA
  2. 2.Department of Dairy ScienceMichigan State UniversityEast LansingUSA
  3. 3.School of Veterinary MedicineUniversity ofIllinoisUrbanaUSA

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