The Immediate Source of the Oviposition-Deterring Pheromone Produced by Larvae of Adalia bipunctata (L.) (Coleoptera, Coccinellidae)

  • E. Laubertie
  • X. Martini
  • C. Cadena
  • M. Treilhou
  • A. F. G. Dixon
  • J.-L. Hemptinne

As is the case for other insects ovipositing on or in resources that are limited in time and/or space, the two-spot ladybird beetle, Adalia bipunctata (L.) produces an oviposition-deterring pheromone (ODP), which is produced by the larval stages. Foraging larvae touch the substrate with their tarsi and the anal disk on the tenth abdominal segment. The aim of this paper was to determine whether the ODP produced by larvae was deposited by the tarsi or the anal disk. Fourth instar larvae either had their anal disk and tarsi, or anal disk, or tarsi coated with a water-soluble mounting medium. Larvae so treated were allowed to walk on filter paper that was subsequently presented to gravid females. The tracks of larvae that had both their tarsi and anal disk masked did not inhibit oviposition. However, the tracks of larvae that had only their tarsi masked significantly inhibited oviposition but those of larvae that had only their anal disk masked did not. It is concluded that the ODP is deposited on the substrate by the anal disk on the tenth abdominal segment of larvae.


oviposition-deterring pheromone larvae anal disk tarsi coccinellidae 



Many thanks are due to Bruno Fréchette, Alexandra Magro and two anonymous referees for constructive discussions that greatly improved the manuscript.


  1. Anbutsu, H., and Togashi, K. (2001). Oviposition deterrent by female reproductive gland secretion in Japanese pine sawyer, Monochamus alternatus. J. Chem. Ecol. 27: 1151–1161.PubMedCrossRefGoogle Scholar
  2. Dempster, J. P. (1997). The role of larval food resource and adult movement in the population dynamics of the orange-tip butterfly (Anthocaris cardamines). Œcologia 111: 549–556.CrossRefGoogle Scholar
  3. Dixon, A. F. G. (1997). Patch quality and fitness in predatory ladybirds. In Dettner, K., Bauer, G., and Völkl, W. (eds.), Vertical Food Web Interactions; Evolutionary Patterns and Driving Forces. Ecological Studies, 130: 205–223.Google Scholar
  4. Dixon, A. F. G. (1998). Aphid Ecology. An Optimization Approach, Chapman and Hall, NY.Google Scholar
  5. Dixon, A. F. G. (2000). Insect Predator-Prey Dynamics. Ladybird Beetles & Biological Control, Cambridge University Press, Cambridge, UK.Google Scholar
  6. Doumbia, M., Hemptinne, J.-L., and Dixon, A. F. G. (1998). Assessment of patch quality by ladybirds: role of larval tracks. Œcologia 113: 197–202.Google Scholar
  7. Ferguson, A. W., Solinhas, M., Ziesman, J., Isidoro, N., Williams, I. H., Scubla, P., Mudd, A., Clark, S. J., and Wadhams, L. J. (1999). Identification of the gland secreting oviposition-deterring pheromone in the cabbage seed weevil, Ceutorhynchus assimilis, and the mechanism of pheromone deposition. J. Insect Physiol. 45: 687–699.PubMedCrossRefGoogle Scholar
  8. Fréchette, B., Alauzet, C., and Hemptinne, J.-L. (2003). Oviposition behaviour of the two-spot ladybird beetle Adalia bipunctata (L.) (Coleoptera, Coccinellidae) on plants marked with conspecific larval tracks. In Soares, A. O., Ventura, M. A., Garcia, V., and Hemptinne, J.-L. (eds.), Proceedings of the 8th International Symposium on Ecology of Aphidophaga: Biology, Ecology and Behaviour of Aphidophagous Insects. Arquipélago—Life and Marine Sciences, (Suppl. 5), pp. 73–77.Google Scholar
  9. Gabel, B., and Thiery, D. (1992). Biological evidence of an oviposition-deterring pheromone in Lobesia botrana Den. Et Schiff. (Lepidoptera, Tortricidae). J. Chem. Ecol. 18: 353–359.CrossRefGoogle Scholar
  10. Godfray, H. C. J. (1994). Parasitoids. Behavioral and Evolutionary Ecology, Princeton University Press, NJ.Google Scholar
  11. Hemptinne, J.-L., Dixon. A. F. G., and Coffin, J. (1992). Aphid attack strategy of ladybird beetles: factors shaping the numerical response. Œcologia, 90: 238–245.Google Scholar
  12. Hemptinne, J.-L., Dixon, A. F. G., Doucet, J.-L., and Petersen, J.-E. (1993). Optimal foraging by hoverflies (Diptera: Syrphidae) and ladybirds (Coleoptera: Coccinellidae): mechanisms. Eur. J. Entomol. 90: 451–455.Google Scholar
  13. Hemptinne, J.-L., Lognay, G., Doumbia, M., and Dixon, A. F. G. (2001). Chemical nature and persistence of the oviposition deterring pheromone in the tracks of the larvae of the two spot ladybird, Adalia bipunctata (Coleoptera: Coccinellidae). Chemoecology 11: 43–47.CrossRefGoogle Scholar
  14. Hodek, I. (1973). Biology of Coccinellidae, Dr. W. Junk, The Hague and Academia, Prague.Google Scholar
  15. Hoffmeister, T. S. (2000). Marking decisions and host discrimination in a parasitoid attacking concealed hosts. Can. J. Zool. 78: 1494–1499.CrossRefGoogle Scholar
  16. Karlson, P., and Lüscher, M. (1959). Pheromones: a new term for a class of biologically active substances. Nature 183: 55–56.PubMedCrossRefGoogle Scholar
  17. Kindlmann, P., and Dixon, A. F. G. (1993). Optimal foraging in ladybird beetles (Coleoptera: Coccinellidae) and ist consequences for their use in biological control. Eur. J. Entomol. 90: 443–450.Google Scholar
  18. Kosaki, A., and Yamaoka, R. (1996). Chemical composition of footprints and cuticula lipids of three species of Lady Beetles. Jpn. J. Appl. Entomol. Zool. 40: 47–53.Google Scholar
  19. Mills, N. (1982). Voracity, cannibalism and coccinellid predation. Ann. Appl. Biol. 101: 144–148.Google Scholar
  20. Nufio, C. R., and Papaj, D. R. (2001). Host marking behavior in phytophagous insects and parasitoids. Entomol. Exp. Appl. 99: 273–293.CrossRefGoogle Scholar
  21. Quiring, D. T., Sweeney, J. W., and Bennett, R. G. (1998). Evidence for a host-marking pheromone in white spruce cone fly, Strobilomyia neanthracina. J. Chem. Ecol. 24: 709–721.CrossRefGoogle Scholar
  22. Ruzicka, Z. (1994). Oviposition-deterring pheromone in Chrysopa oculata (Neuroptera: Chrysopidae). Eur. J. Entomol. 91: 361–370.Google Scholar
  23. Ruzicka, Z. (1996). Oviposition-deterring pheromone in chrysopids: Intra- and interspecific effects. Eur. J. Entomol. 93: 161–166.Google Scholar
  24. Ruzicka, Z. (2002). Persistence of deterrent larval tracks in Coccinella septempunctata, Cycloneda limbifer and Semiadalia undecimnotata. Eur. J. Entomol. 99: 471–475.Google Scholar
  25. Ruzicka, Z. (2003). Perception of oviposition-deterring larval tracks in aphidophagous coccinellids Cycloneda limbifer and Ceratomegilla undecimnotata. Eur. J. Entomol. 100: 345–350.Google Scholar
  26. Ruzicka, Z., and Havelka, J. (1998). Effects of oviposition-deterring pheromone and allomones on Aphidoletes aphidimyza (Diptera: Cecidomyiidae). Eur. J. Entomol. 95: 211–216.Google Scholar
  27. Spiegler, P. E. (1962). The origin and the nature of the adhesive substance in larvae of the genus Chrysopa (Neuroptera, Chrysopidae). Ann. Entomol. Soc. Am. 55: 69–77.Google Scholar
  28. Städler, E., Ernst, B., Hurter, J., and Boller, E. (1994). Tarsal contact chemoreceptor for the host marking pheromone of the cherry fruit fly, Rhagoletis cerasi: responses to natural and synthetic compounds. Physiol. Entomol. 19: 139–151.CrossRefGoogle Scholar
  29. Strong, D. R., Lawton, J. H., and Southwood, R. (1984). Insects on Plants. Community Patterns and Mechanisms, Blackwell Scientific Publications, Oxford.Google Scholar
  30. Wigglesworth, V. B. (1972). The Principles of Insect Physiology, Chapman and Hall, NY.Google Scholar
  31. Zar, J. H. (1996). Biostatistical Analysis (3rd edn), Prentice Hall International Editions, Upper Saddle River, NJ.Google Scholar

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • E. Laubertie
    • 1
  • X. Martini
    • 1
  • C. Cadena
    • 2
  • M. Treilhou
    • 2
  • A. F. G. Dixon
    • 3
  • J.-L. Hemptinne
    • 1
    • 4
  1. 1.UMR CNRS/UPS/ENFA 5174 “Evolution et diversité biologique,” Ecole nationale de Formation agronomiqueCastanet-Tolosan CedexFrance
  2. 2.Unité propre “Signaux biologiques et métabolites secondaires,” Ecole nationale de Formation agronomiqueCastanet-Tolosan CedexFrance
  3. 3.School of Biological Sciences, University of East AngliaNorwichUK
  4. 4.Ecole nationale de Formation agronomiqueCastanet-Tolosan CedexFrance

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