Innovative diagnostic tools, new approaches, and novel methodologies for monitoring and quantification of biological biomarkers have become a dominant challenge. In this work, a novel fast, facile, accurate, selective, and ultra-sensitivity optical approach for troponin I (cTn) cardiac biomarker. cTn, is used as an early diagnostic test for the patients of myocardial infarction. It can support the accurate decisions in the absolute necessity cases. Herein, a manganese metal–organic framework “Mn-MOF” was synthesized via a facile route. The structural and morphology of the produced MOF was confirmed using several tools of characterizations. The chemical stability and photoluminescence (PL) studies of the Mn-MOF were investigated to elucidate the availability of using Mn-MOF as optical biosensor for cTn. Mn-MOF exhibited a characteristic photoluminescence (PL) emission (λem = 422 nm) that was blue-shifted and encountered a remarkable reduction in intensity in presence of cTn (i.e., PL quenching agent). According to Stern-Völmer equation, a linear [cTn]-quenching relationship was observed over a wide concentration range of cTn (1.0 fg- 30.0 pg/mL) and linear range between 1.0 fg and 0.025 ng/mL. The limit of quantitation (LOQ) and detection (LOD) for the method ware estimated to be 30.0 fg/mL and 10.0 fg/mL, respectively. Based on the present optical approach; the Mn-MOF can be used successfully as a cTn-biosensor in biological samples, even the presence of different interfering analytes. The mechanism of quenching was studied, and the results revealed that the dynamic type was achieved.
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This project was funded by the Deanship of Scientific Research (DSR) at King Abdulaziz University, Jeddah, Saudi Arabia under grant no. DF-405-247-1441. The authors, therefore, acknowledge with thanks DSR for technical and financial support.
Conflict of interest
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Statement of Human and Animal Rights
The human patient samples used in this study were provided by the Family Medical Laboratory, Ministry of Health, Cairo, Egypt. Informed consent was obtained. The studies were approved by the appropriate ethics committee (Ministry of Health, Egypt) and were performed in accordance with ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki Declaration of 1975, as revised in 2000 and 2008 ethical standards. We did not use the samples in any research involving human participants or research involving physical interventions on study participants or involving processing of personal data but conducted the research according to the method described in the article.
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Basaleh, A.S., Sheta, S.M. Manganese Metal–Organic Framework: Chemical Stability, Photoluminescence Studies, and Biosensing Application. J Inorg Organomet Polym (2021). https://doi.org/10.1007/s10904-021-01888-4
- Cardiac troponin
- Metal–organic frameworks
- Chemical stability
- Quenching mechanism