Dodonaea viscosa Leaf Extract Assisted Synthesis of Gold Nanoparticles: Characterization and Cytotoxicity Against A549 NSCLC Cancer Cells

  • M. Anandan
  • H. Gurumallesh Prabu


The gold nanoparticles with distinct morphologies were synthesized using Dodonaea viscosa leaf extract in different polar/nonpolar solvents. The solvents such as methanol, acetone, acetonitrile and water were used in the synthesis of nanoparticles which attributed to obtaining nanoparticles with different physical, chemical and cytotoxic properties. The FT-IR results evidenced the presence of functional groups responsible for effective reduction (Au3+ to Au0) and capping activity. The XRD and EDX results confirmed the formation of gold nanoparticles whereas the SEM and TEM results revealed their distinct size (58, 90, 43 and 30 nm) and morphology (spherical particles, irregularly shaped particles and distorted spherical particles) for different solvents (methanol, acetone, acetonitrile and water) used for extraction. The IC50 values of synthesized AuNPs were found to be 4, 60, 8, and 100 µg/ml of leaf extracts obtained from methanol, acetone, acetonitrile and water, respectively. The cytotoxicity of AuNPs revealed that the synthesized AuNPs could inhibit the growth of A549 NSCLC cells effectively and activity of the NPs varies with distinct morphology.

Graphical Abstract


A549 NSCLC cancer cells Cytotoxicity Dodonaea viscosa Gold nanoparticles HR-TEM 



The authors are thankful to the University Grants Commission (UGC-BSR: F.4-1/2006 (BSR)/7-188/2007 (BSR)), New Delhi, India for the financial assistance by the scheme of a basic scientific research fellowship. The author (M. Anandan) acknowledges the Department of Industrial Chemistry and the Department of Physics, Alagappa University, Karaikudi for providing Scanning Electron Microscopy facility and X-Ray Diffraction analysis facility, respectively. The author (M. Anandan) is very grateful to Mr. Varunkumar, Research scholar, Cancer Biology lab, Department of Biochemistry, Bharathidasan University, Trichy for his help to carry out cancer studies.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Industrial Chemistry, School of Chemical SciencesAlagappa UniversityKaraikudiIndia

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