Cross-Sectional TEM Analysis of an ITO Surface Coated with Photosystem I and Molecular Wires
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Photosystem I (PSI) from Thermosynechococcus elongatus was immobilized on ITO by using vitamin K1 exchange with artificial molecular wires in PSI. A PSI-modified surface was prepared for observation of lateral TEM images to provide an understanding of its interfacial structure. This paper describes the use of an ultramicrotome to prepare ultra-thin (< 20 nm) sections of PSI on ITO substrates. Cross-sectional TEM and energy-dispersive X-ray spectrometry supported that monolayer PSI was attached to the ITO electrode.
KeywordsCross-sectional TEM Photosynthesis Photosystem Ultramicrotome Energy-dispersive X-ray spectrometry
The authors thank Dr. Osamu Oishi of the Institute for Molecular Science for technical assistance with sample preparation and observation by TEM and EDS. The present work was financially in part supported by Core Research for Evolutional Science and Technology (CREST) from JST, Tokyo Kasei Chemical Promotion foundation, Nippon Sheet Glass Foundation for Materials Science and Engineering, Precise Measurement Technology Promotion Foundation, Grant-in-Aids for Scientific Research (C) (No. 15K05604), Grant-in-Aids for Scientific Research (S) (No. 26220801), and Scientific Research on Innovative Areas “Molecular Architectonics: Orchestration of Single Molecules for Novel Functions” (area 2509, Nos. 26110505, 26110506, 16H00957, and 16H00958) from the Ministry of Education, Culture, Sports, Science, and Technology, Japan.
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