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Ultrasensitive Electrochemiluminescence Immunoassay for Protein Specific Detection Based on Dendrimer-Encapsulated Gold Nanoparticles Labels

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Abstract

A sensitivity-enhanced electro chemiluminescence immunoassay (ECLIA) was fabricated by covalently immobilizing a monoclonal prostate specific antigen (PSA) antibody (anti-PSA, Ab2) and a luminophore (luminol) on dendrimer-encapsulated gold nanoparticles (Den/AuNPs) as electrochemiluminescence labels. The primary antibody was immobilized on Fe3O4@SiO2 NP support, and the antibody-loaded Fe3O4@SiO2 NP was placed onto an indium tin oxide working electrode in a home made electrochemiluminescence (ECL) cell. PSA and Ab2/Luminol/Den/AuNP was successively injected into the cell, and conjugated to form a sandwich-type immunocomplex. Under optimized experimental conditions, the proposed ECLIA provided a linear response range from 0.001 to 100.0 ng/mL with a low detection limit of 0.3 pg/mL. The PSA assay results in clinical serum samples were in good agreement with the commercially available electro chemiluminescence assay. The ECL immunosensor has the advantages of high sensitivity, specificity and stability and may be a promising technique for tumor marker detection.

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Acknowledgments

This work was financially supported by the Natural Science Research Foundation of China (21207048), Major State Basic Research Development Program of China (973 Program) (no. 2010CB933504), Natural Science Foundation of Shandong Province, China (ZR2011BQ019), Youth Science and Technology Jinan star program (20110102) and State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences (KF2011-03).

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Authors and Affiliations

  1. School of Chemistry & Chemical Engineering, Technology Research Center for Colloidal Materials, Shandong University, Jinan, 250100, China

    Shenguang Ge, Xiuling Jiao & Dairong Chen

  2. Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, University of Jinan, Jinan, 250022, China

    Jinghua Yu

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  1. Shenguang Ge
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  2. Jinghua Yu
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  3. Xiuling Jiao
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  4. Dairong Chen
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Correspondence to Dairong Chen.

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Ge, S., Yu, J., Jiao, X. et al. Ultrasensitive Electrochemiluminescence Immunoassay for Protein Specific Detection Based on Dendrimer-Encapsulated Gold Nanoparticles Labels. J Inorg Organomet Polym 23, 1113–1121 (2013). https://doi.org/10.1007/s10904-013-9895-9

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  • DOI: https://doi.org/10.1007/s10904-013-9895-9

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