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Probing the Interaction of Newly Synthesized Pt(II) Complex on Human Serum Albumin Using Competitive Binding Site Markers

Journal of Fluorescence volume 29, pages 827–835 (2019)Cite this article

Abstract

Considering the importance of pharmacology and the influence of drugs on biological materials, the effects of a newly designed and synthesized platin complex (2,2′-Bipyridine-3,3′-dicarboxylic acid, oxalato Platinum(II), as an antitumor drug was tested on the structure of blood carrier protein of human serum albumin (HSA) using various spectroscopic techniques including UV-visible, fluorescence, and circular dichroism at 25 and 37 °C. Results of the fluorescence measurements revealed that adding the complex caused reduction in intrinsic fluorescence emission of HSA resulted from dynamic quenching of HSA. The number of binding sites and binding constants were calculated at both temperatures of 25 and 37 °C. In addition, in order to identify the complex’s binding site on HSA employing spectroscopy, the competitive studies were followed using warfarin, digitoxin and ibuprofen as site markers of Sudlow sites I, II and III. Competitive binding test results have shown that Pt(II) complex bind on the warfarin binding site (or Sudlow sites I) on HSA. Besides, a reduction in thermal stability for HSA was observed in the presence of the newly designed Pt(II) complex.

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Acknowledgments

Authors thank the Research Council of Kharazmi University for financial support of this work.

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Authors and Affiliations

  1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Akram Najaran & Nasim Hayati Roodbari

  2. Department of Cell & Molecular Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran

    Adeleh Divsalar

  3. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

    Ali Akbar Saboury

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  1. Akram Najaran
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  2. Adeleh Divsalar
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  3. Ali Akbar Saboury
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  4. Nasim Hayati Roodbari
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Correspondence to Adeleh Divsalar.

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Najaran, A., Divsalar, A., Saboury, A.A. et al. Probing the Interaction of Newly Synthesized Pt(II) Complex on Human Serum Albumin Using Competitive Binding Site Markers. J Fluoresc 29, 827–835 (2019). https://doi.org/10.1007/s10895-019-02383-3

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  • DOI: https://doi.org/10.1007/s10895-019-02383-3

Keywords

  • Site markers
  • HSA
  • Sudlow sites
  • Competitive studies
  • Fluorescence quenching