A Novel Water-soluble Ratiometric Fluorescent Probe Based on FRET for Sensing Lysosomal pH
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A new ratiometric fluorescent probe based on Förster resonance energy transfer (FRET) for sensing lysosomal pH has been developed. The probe (RMPM) was composed of imidazo[1,5-α]pyridine quaternary ammonium salt fluorophore as the FRET donor and the rhodamine moiety as the FRET acceptor. It’s the first time to report that imidazo[1,5-α]pyridine quaternary ammonium salt acts as the FRET donor. The ratio of fluorescence intensity of the probe at two wavelengths (I424/I581) changed significantly and responded linearly toward minor pH changes in the range of 5.4–6.6. It should be noted that it’s rare to report that a ratiometric pH probe could detect so weak acidic pH with pKa = 6.31. In addition, probe RMPM exhibited excellent water-solubility, fast-response, all-right selectivity and brilliant reversibility. Moreover, RMPM has been successfully applied to sensing lysosomal pH in HeLa cells and has low cytotoxicity.
KeywordsRatiometric fluorescent probe Water-solubility Imidazo[1,5-α]pyridine quaternary ammonium salt Lysosomal pH Image
This study is supported by the Natural Science Foundation of Shandong Province (ZR2014BM004) and (ZR2013BM020).
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