High Sensitivity Bacillus thuringiensis Cry1Ac Protein Detections Using Fluorescein Diacetate Nanoparticles
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A highly sensitive transgenic protein analysis method was proposed here based on fluorescein diacetate (FDA). First, FDA was prepared by the ball mill to harvest the nano-sized organic particles. Further examines showed that the FDA size can be controlled by the speed of centrifugation which can obtain FDA in well-distributed size. Cy3 antibody immobilization tests showed that the proteins can attach onto the FDA particles while keep bioactivities. FDA and Cry1Ac antibody immunoassay tests showed that when the FDA particle was in 150 nm, the linear range was 0.01 ng/L-30 μg/mL. And it has the lower detection limitation of 0.01 ng/L, which is 100 times more sensitive than the ELISA methods. These results indicate that the FDA related immunoassays are the promising approach in the transgenic analysis.
KeywordsFluorescein diacetate Fluorescent analysis Cry1Ac protein Immunoassay
The Project was sponsored by the Scientific Research Initial funding for the advanced talent of Janghan University (08010001, 06660001, 2011023), Hubei Province Innovative Young Research Team in Universities (T201318), Hubei Provincial Key Natural Science Foundations (2014CFA098) and National High Technology Research and Development Program of China ( 863 Program: 2015AA033406).
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