Rapid Fluorescence Spectroscopic Characterization of Salivary DNA of Normal Subjects and OSCC Patients Using Ethidium Bromide
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Recently, deoxyribonucleic acid (DNA) based biomarker(s) detection has been employed for cancer diagnosis. Earlier reports have suggested the presence of more DNA in the saliva of oral squamous cell carcinoma (OSCC) than normal by electrophoresis technique. Based on these, steady state and excited state kinetics of salivary DNA has been performed with 27 normal subjects and 67 OSCC patients saliva using ethidium bromide as a probe to look for the possibility in discrimination between them. On statistical analysis the sensitivity and specificity of 88.9 and 94.0 % has been achieved from the fluorescence emission spectra and 88.9 and 92.5 % with that of fluorescence excitation.
KeywordsSaliva mass screening Salivary DNA Ethidium bromide Fluorescence steady and excited state kinetics Simple and rapid technique
The work was supported by DAE-BRNS Grant (Ref. No. 2009/34/38), Govt. of India. The author, M. Yuvaraj acknowledges DST-PURSE for fellowship. We acknowledge Dr. S. Sri Priya and Mrs. Arivazhagi of Government Arignar Anna Cancer Hospital, Regional Cancer Centre, Karapettai, Kanchipuram, for providing the samples and the histopathological reports.
- 1.The international statistical classification of diseases and related health problems (1992) Geneva. World Health Organization 10th EdGoogle Scholar
- 2.Cancer Facts and Figures (2007) Atlanta. American Cancer SocietyGoogle Scholar
- 3.Ravindran R, Deepa MG (2012) Salivary tumour markers in oral cancer: brief review. Oral Maxillofac Pathol J 2:238–244Google Scholar
- 5.Coelho KR (2012) Challenges of the oral cancer burden in India. J Cancer Epidemol Article ID 701932: 1–17. doi: 10.1155/2012/701932
- 22.Keskinova E, Angelov D, Angelova SV, Shaqiri Z, Veseli A (1998) Effects of high-intensity UV radiation on isolated and DNA-intercalated ethidium bromide. Fizika A 7:17–26Google Scholar