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Location of Novel Benzanthrone Dyes in Model Membranes as Revealed by Resonance Energy Transfer

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Abstract

Förster resonance energy transfer (FRET) between anthrylvinyl-labeled phosphatidylcholine (AV-PC) as a donor and newly synthesized benzanthrones (referred to here as A8, A6, AM12, AM15 and AM18) as acceptors has been examined to gain insight into molecular level details of the interactions between benzanthrone dyes and model lipid membranes composed of zwitterionic lipid phosphatidylcholine and its mixtures with anionic lipids cardiolipin (CL) and phosphatidylglycerol (PG). FRET data were quantitatively analyzed in terms of the model of energy transfer in two-dimensional systems taking into account the distance dependence of orientation factor. Evidence for A8 location in phospholipid headgroup region has been obtained. Inclusion of CL and PG into PC bilayer has been found to induce substantial relocation of A6, AM12, AM15 and AM18 from hydrophobic membrane core to lipid-water interface.

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Acknowledgments

This work was supported by the grant from Fundamental Research State Fund (project number F.54.4/015) and CIMO Fellowship (OZ).

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Authors and Affiliations

  1. Department of Nuclear and Medical Physics, V.N. Karazin Kharkiv National University, 4 Svobody Sq., Kharkiv, 61077, Ukraine

    Olga Zhytniakivska, Valeriya Trusova & Galyna Gorbenko

  2. Department of Chemistry and Geography, Faculty of Natural Science and Mathematics, Daugavpils University, 13 Vienibas, Daugavpils, LV5401, Latvia

    Elena Kirilova, Inta Kalnina & Georgiy Kirilov

  3. Institute of Bioorganic Chemistry, Russian Academy of Science, 16/10 Miklukho-Maklaya, Moscow, 117871, Russia

    Julian Molotkovsky

  4. Department of Biomedical Engineering and Computational Science, School of Science and Technology, Aalto University, FI-00076, Espoo, Finland

    Jukka Tulkki & Paavo Kinnunen

  5. 52-52 Tobolskaya Str., Kharkiv, 61072, Ukraine

    Olga Zhytniakivska

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  1. Olga Zhytniakivska
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  2. Valeriya Trusova
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Correspondence to Olga Zhytniakivska.

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Zhytniakivska, O., Trusova, V., Gorbenko, G. et al. Location of Novel Benzanthrone Dyes in Model Membranes as Revealed by Resonance Energy Transfer. J Fluoresc 24, 899–907 (2014). https://doi.org/10.1007/s10895-014-1370-7

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  • DOI: https://doi.org/10.1007/s10895-014-1370-7

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