Resveratrol Interferes with Fura-2 Intracellular Calcium Measurements
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Resveratrol, a naturally occurring polyphenol found in some fruits and especially in grapes, has been reported to provide diverse health benefits. Resveratrol’s mechanism of action is the subject of many investigations, and some studies using the ratiometric calcium indicator Fura-2 suggest that it modulates cellular calcium responses. In the current study, contradictory cellular calcium responses to resveratrol applied at concentrations exceeding 10 μM were observed during in vitro imaging studies depending on the calcium indicator used, with Fura-2 indicating an increase in intracellular calcium while Fluo-4 and the calcium biosensor YC3.60 indicated no response. When cells loaded with Fura-2 were treated with 100 μM resveratrol, excitation at 340 nm resulted in a large intensity increase at 510 nm, but the expected concurrent decline with 380 nm excitation was not observed. Pre-treatment of cells with the calcium chelator BAPTA-AM did not prevent a rise in the 340/380 ratio when resveratrol was present, but it did prevent an increase in 340/380 when ATP was applied, suggesting that the resveratrol response was an artifact. Cautious data interpretation is recommended from imaging experiments using Fura-2 concurrently with resveratrol in calcium imaging experiments.
KeywordsCa2+ signaling Fura-2 Fluo-4 YC3.60 BAPTA-AM Resveratrol
This work was supported by a Basic Science Award from the American Diabetes Association to CAL and NIH grants R01 DK074966 and R01 DK092616 to MWR. Adenovirus YC3.60 was a generous gift from Dr. Christopher Rhodes, University of Chicago, Chicago, IL.
- 3.Park S, Ahmad F, Philp A, Baar K, Williams T, Luo H, Ke H, Rehmann H, Taussig R, Brown AL, Kim MK, Beaven MA, Burgin AB, Manganiello V, Chung JH (2012) Resveratrol ameliorates aging-related metabolic phenotypes by inhibiting cAMP phosphodiesterases. Cell 148:421PubMedCentralPubMedCrossRefGoogle Scholar
- 4.Jakab M, Lach S, Bacova Z, Langeluddecke C, Strbak V, Schmidt S, Iglseder E, Paulmichl M, Geibel J, Ritter M (2008) Resveratrol inhibits electrical activity and insulin release from insulinoma cells by block of voltage-gated Ca2+ channels and swelling-dependent Cl- currents. Cell Physiol Biochem 22:567PubMedCrossRefGoogle Scholar