Journal of Fluorescence

, Volume 23, Issue 5, pp 921–927 | Cite as

Interaction Mechanism of Trp-Arg Dipeptide with Calf Thymus DNA

  • Jing Lin
  • Canzhu Gao
  • Rutao Liu


The interaction between Trp-Arg dipetide (WR) and calf thymus DNA (ctDNA) in pH 7.4 Tris-HCl buffer was investigated by multi-spectroscopic techniques and molecular modeling. The fluorescence spectroscopy and UV absorption spectroscopy indicated that WR interacted with ctDNA in a minor groove binding mode and the binding constant was 4.1 × 103. The ionic strength effect and single-stranded DNA (ssDNA) quenching effect further verified the minor groove binding mode. Circular dichroism spectroscopy (CD) was employed to measure the conformation change of ctDNA in the presence of WR. The molecular modeling results illustrated that electrostatic interaction and groove binding coexisted between them and the hydrogen bond and Van der Waals were main acting forces. All the above methods can be widely used to investigate the interaction of peptide with nucleic acids, which contributes to design the structure of new and efficient drugs.


WR interacted with DNA in electrostatic and minor groove binding mode, which resulted in the conformational and micro-environmental changes of DNA.


Trp-Arg ct DNA Interaction mechanisms Spectroscopic Molecular modeling 



The work is supported by NSFC (21277081), the Cultivation Fund of the Key Scientific and Technical Innovation Project, Ministry of Education of China (708058), and Independent innovation foundation of Shandong University natural science projects (2012DX002) are also acknowledged.


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, China-America CRC for Environment & HealthShandong UniversityJinanPeople’s Republic of China
  2. 2.School of Environmental Science and EngineeringShandong UniversityJinanPeople’s Republic of China

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