Study on Synthesis and Spectrum of Novel Styryl Cyanine Dyes with a Carbazole Bridged Chain
- 485 Downloads
Based on the frequently-used cyanine dye probe thiazole orange (TO), a novel kind of cyanine dye was designed and synthesized. Carbazole was inserted into the methylidyne structure of TO as a bridge to afford a kind of novel styryl cyanine dye with carbazole bridged chain. The dyes were characterized by HNMR and MS. The spectra of the novel dyes were also studied and the results showed that the fluorescent wavelength of novel carbzole dye shifted red for 100 nm, stock shift increased by 70 nm and the fluorescent intensity enhanced by 70 times compared to that of TO. When the novel dye was labeled by BSA, its fluorescent wavelength changed little and the intensity enhanced. It is indicated that it can be used as an excellent fluorescent probe in biological labeling.
KeywordsCarbazole Bridge chain Novel styryl cyanine dye Fluorescence Probe
This work was financially supported by the National Natural Science Foundation of China (No. 21072147, 51178289), Key Project of Science and Technology Committee of Tianjin (10JCYBJC10500). The work in the article also supported by Technology Development Foundation Plan Project of Tianjin Colleges(No. 20100503).
- 2.Zhang PQ et al (2008) Synthesis, antioxidation activity of 5-[2-(8-Hydroxy-quinolin-2-yl)-vinyl]-2-methyl-quinolin-8-ol and its induction proliferation of mesenchymal stem cells. Chin J Org Chem 28(6):1035–1039Google Scholar
- 3.Wang GR et al (2009) Synthesis, antioxidation activity of (E)-9-p-Tolyl-3-[2-(8-hydroxy-qui-nol-2-yl)vinyl]-carbazole and (E)-9-(p-Anisyl)-3-[2-(8-hydroxy-qui-nol-2-yl)vinyl]-carbazole and their induction proliferation of mesenchymal stem cells. Acta Chimica Sinica 67(9):974–982Google Scholar
- 21.Gu YC et al (2010) Synthesis, crystal structure and spectral properties of thiazole orange derivative. Chalcogenide Lett 7(5):305–312Google Scholar