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Water Deficit and Salt Stress Diagnosis Through LED Induced Chlorophyll Fluorescence Analysis in Jatropha curcas L.

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Abstract

LED induced chlorophyll fluorescence analysis is employed to investigate the effect of water deficit and salt stress upon the growth process of Jatropha curcas L.. Red(Fr) and far-red(FFr) chlorophyll fluorescence around 685 nm and 735 nm, respectively, were observed and examined as a function of the stress intensity(salt concentration and water deficit). The fluorescence ratio Fr/FFr which is a valuable nondestructive and nonintrusive indicator of the chlorophyll content of leaves was exploited to monitor the jatropha plants under stress. The data indicated that salinity plays a minor role in the chlorophyll concentration of leaves for NaCl concentrations in the 25 to 200 mM range. The fluorescence ratio also permitted the detection of damage caused by water deficit in the early stages of the plants growing process. A significant variation of the Fr/FFr ratio was observed in the first 10 days of the experiment, and before signs of visual stress became apparent. The results suggest that the Fr/FFr ratio is an early-warning indicator of water deficit stress

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Acknowledgements

The financial support for this research by CNPq-Conselho Nacional de Desenvolvimento Científico e Tecnológico(Proc.472328/2008-05) and FACEPE(Fundação de Amparo a Ciência e Tecnologia do Estado de Pernambuco) APQ 0504–1.05/08, Instituto Nacional de Ciência e Tecnologia(INFOS) is gratefully acknowledged. Elias A. da Silva Jr. is supported by a graduate studentship from FACEPE. Mr. Ronaldo Alves is supported by an undergraduate studentship from the PIBIC/UFRPE.

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Authors and Affiliations

  1. Departamento de Física, Universidade Federal Rural de Pernambuco, Recife, Brazil

    E. A. Silva Jr, A. S. Gouveia-Neto, D. S. Moura & E. B. Costa

  2. Departamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife, Brazil

    R. A. Oliveira & P. C. Cunha

  3. Departamento de Química, Universidade Federal Rural de Pernambuco, Recife, Brazil

    T. J. R. Câmara

  4. Departamento de Biologia, Universidade Federal Rural de Pernambuco, Recife, Brazil

    L. G. Willadino

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  1. E. A. Silva Jr
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  2. A. S. Gouveia-Neto
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  3. R. A. Oliveira
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Correspondence to A. S. Gouveia-Neto.

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Silva, E.A., Gouveia-Neto, A.S., Oliveira, R.A. et al. Water Deficit and Salt Stress Diagnosis Through LED Induced Chlorophyll Fluorescence Analysis in Jatropha curcas L.. J Fluoresc 22, 623–630 (2012). https://doi.org/10.1007/s10895-011-0998-9

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