Journal of Fluorescence

, Volume 22, Issue 2, pp 557–564 | Cite as

Determination of Ofloxacin using a Highly Selective Photo Probe Based on the Enhancement of the Luminescence Intensity of Eu3+—Ofloxacin Complex in Pharmaceutical and Serum Samples

  • M. S. Attia
  • Amr A. Essawy
  • A. O. Youssef
  • Marwa S. Mostafa
Original Paper


A rapid, simple and sensitive spectrofluorimetric method for determination of trace amount of ofloxacin was developed. At pH 5.1 the ofloxacin enhances the luminescence intensity of the Eu3+ ion in Eu3+- ofloxacin complex at λex = 365 nm. The produced luminescence intensity of Eu3+-ofloxacin complex was in proportional to the concentration of ofloxacin. The working range for the determination of ofloxacin was 5.0 × 10-9–5.0 × 10-6 mol L-1 with lower detection limit (LOD) and quantitative detection limit (QDL) of 3 × 10-9 and 9 × 10-9 mol L-1, respectively. The enhancement mechanism of the luminescence intensity in the Eu3+-ofloxacin system has been also explained. The method revealed good selectivity for ofloxacin in the presence of coexisting substances and used successfully for the assay of ofloxacin in pharmaceutical preparations and serum. A comparison with other standard methods was also discussed.


Ofloxacin Europium Luminescence Enhancement Energy transfer Photo probe 


  1. 1.
    Goodman A, Goodman LS, Hall TW, Murad F (1989) Las Bases Farmacolo´gicas de la Terape´utica, 7th edn. Panamericana, Madrid, pp 1132–1136Google Scholar
  2. 2.
    Kapetanovic V, Milavanovic L, Erceg M (1996) Spectrophotometric and polarographic investigation of the ofloxacin–Cu (II) complexes. Talanta 43:2123–2130PubMedCrossRefGoogle Scholar
  3. 3.
    Mathur SC, Kumar Y, Murugesan N, Rathore Y, Sethi PD (1992) Spectrophotometric determination of ofloxacin in pharmaceutical formulations. Indian Drugs 29:376–377Google Scholar
  4. 4.
    Sastry CS, Rama Rao K, Prasad D (1995) Extractive spectrophotometric determination of some fluoroquinolone derivatives in pure and dosage forms. Talanta 42:311–316PubMedCrossRefGoogle Scholar
  5. 5.
    Issa YM, Abdel-Gawad FM, Abou Table MA, Hussein HM (1997) Spectrophotometric determination of ofloxacin and lomefloxacin hydrochloride with some sulphonphthalein dyes. Anal Lett 30:2071–2084CrossRefGoogle Scholar
  6. 6.
    Panzade PD, Mahadik KR (2001) Simultaneous estimation of ofloxacin and tinidazole in tablet dosage form. Indian Drugs 38:368–370Google Scholar
  7. 7.
    Rao Y, Tong Y, Zhang XR, Lu GA, Baeyens WR (2000) Determination of ofloxacim using a chemiluminescence flow-injection method. Anal Chim Acta 416:227–230CrossRefGoogle Scholar
  8. 8.
    Aly FA, Al-Tamimi SA, Alwarthan AA (2001) Chemiluminescence determination of some fluoroquinolone derivatives in pharmaceutical formulations and biological fluids using [Ru(bipy)32 C]-Ce(IV) system. Talanta 53:885–893PubMedCrossRefGoogle Scholar
  9. 9.
    Le Coguic A, Bidault R, Farinotti R, Dauphin A (1988) Determination of ofloxacin in plasma and urine by liquid chromatography. J Chromatogr 434:320–323PubMedCrossRefGoogle Scholar
  10. 10.
    Okazaki O, Aoki H, Hakusui H (1991) High-performance liquidchromatographic determination of (S)-(K) ofloxacin and its metabolites in serum and urine using a solid-phase cleanup. J Chromatogr 563:313–322PubMedCrossRefGoogle Scholar
  11. 11.
    Carlucci G, Mazzeo P, Fantozzi T (1993) Determination of ofloxacin in pharmaceutical forms by high-performance liquid chromatography and derivative UV-spectrophotometry. Anal Lett 26:2193–2201CrossRefGoogle Scholar
  12. 12.
    Shinde VM, Desai BS, Tendolkar NM (1998) Selective determination of fluoroquinolone derivatives from tablets by reverse-phase HPLC. Indian Drugs 35:715–717Google Scholar
  13. 13.
    Halkar UP, Ankalkope PB (2000) Reverse phase high-performance liquid chromatographic determination of ofloxacin and tinizadole in tablets. Indian Drugs 37:585–588Google Scholar
  14. 14.
    Zhang SS, Liu HX, Yuan ZB, Yu CL (1998) A reproducible, simple and sensitive high-performance capillary electrophoresis method for simultaneous determination of capreomycin, ofloxacin and pasiniazide in urine. J Pharm Biomed Anal 17:617–622PubMedCrossRefGoogle Scholar
  15. 15.
    Horstkoetter C, Blaschke G (2001) Stereoselective determination of ofloxacin and its metabolites in human urine by capillary electrophoresis using laser-induced fluorescence detection. J Chromatogr B754:169–178Google Scholar
  16. 16.
    Zhang SS, Liu H, Wu YJ, Yu CL (2001) On-column amperometric detection of ofloxacin and pasiniazid in urine by capillary electrophoresis with and improved fractured joint and small detection cell. Analyst 126:441–445PubMedCrossRefGoogle Scholar
  17. 17.
    Tamer A (1990) Adsorptive stripping voltammetric determination of ofloxacin. Anal Chim Acta 231:129–131CrossRefGoogle Scholar
  18. 18.
    Ambrosi A, Antiochia R, Campanella L, Dragone R, Lavagnini I (2005) Electrochemical determination of pharmaceuticals in spiked water samples. J Hazard Mater 122:219–225PubMedCrossRefGoogle Scholar
  19. 19.
    Zhou G, Pan J (1995) Polarographic and voltammetric behavior of ofloxacin and its analytical application. Anal Chim Acta 307:49–53CrossRefGoogle Scholar
  20. 20.
    Rizk M, Belal F, Aly F, El-Enany N (1998) Differential pulse polarographic determination of ofloxacin in pharmaceuticals and biological fluids. Talanta 46:83–89PubMedCrossRefGoogle Scholar
  21. 21.
    Wu J, Zhao H, Wei L, Ai TZ, Dong XZ (2001) Preparation and application of a poly(vinyl-chloride) membrane coated glass electrode-based ofloxacin ISE. Fenxi Huaxue 29:1106Google Scholar
  22. 22.
    El-Yazbi FA (1992) Spectrophotometric and spectrofluorimetric determination of ofloxacin. Spectrosc Lett 25:279–291CrossRefGoogle Scholar
  23. 23.
    Ballesteros O, Vílchez JL, Navalón A (2002) Determination of the antibacterial ofloxacin in human urine and serum samples by solid-phase spectrofluorimetry. J Pharm Biomed Anal 30:1103–1110PubMedCrossRefGoogle Scholar
  24. 24.
    Gong QJ, Quiao JL, Du LM, Dong C (2000) Recognition and simultaneous determination of ofloxacin enantiomers by synchronization first derivative fluorescence spectroscopy. Talanta 53:359–365PubMedCrossRefGoogle Scholar
  25. 25.
    Benesi HA, Hildebrand JH (1949) J Am Chem 71:2703CrossRefGoogle Scholar
  26. 26.
    Attia MS (2009) Spectrochim Acta Part A 74:972–976CrossRefGoogle Scholar
  27. 27.
    Attia MS (2010) J Pharm Biomed Anal 51:7–11PubMedCrossRefGoogle Scholar
  28. 28.
    Attia MS, Bakir E, Abdel-aziz AA, Abdel-mottaleb MSA (2011) Talanta 84:27–33PubMedCrossRefGoogle Scholar
  29. 29.
    Attia MS, Othman AM, Elraghi E, Aboul-Enein HY (2011) J Fluoresc. doi: 10.1007/s10895-010-0764-4
  30. 30.
    International Conference on Hormonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use, ICH Harmonised Tripartite Guideline, Validation of Analytical Procedures: Text and Methodology Q2(R 1), Complementary Guideline on Methodology dated 06 November 1996, incorporated in November 2005, LondonGoogle Scholar
  31. 31.
    British Pharmacopoeia (1999) vol. II, Her Majesty’s Stationary Office, London, p 2705Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • M. S. Attia
    • 1
  • Amr A. Essawy
    • 2
  • A. O. Youssef
    • 1
  • Marwa S. Mostafa
    • 1
  1. 1.Chemistry DepartmentAin Shams UniversityCairoEgypt
  2. 2.Chemistry DepartmentFayoum UniversityAl FayoumEgypt

Personalised recommendations