Ultrasensitive Study of Gatifloxacin Based on Its Enhancing Effect on the Cerium (IV)-Sodium Hyposulfite Chemiluminescence Reaction in a Micellar Medium
- 146 Downloads
A sensitive and rapid flow-injection chemiluminescence (CL) method has been developed for the determination of gatifloxacin in pharmaceutical preparations and biological samples. The method is based on the enhancing effect of gatifloxacin on CL emission generated by the interaction of Ce (IV) in sulphuric acid and sodium hyposulphite (Na2S2O4) sensitized by sodium dodecyl benzene sulfonate (SDBS). Strong CL emission was observed when gatifloxacin was injected into the Ce (IV) in sulphuric acid and Na2S2O4 solution incorporated with SDBS in a flow-cell. Several experimental parameters affecting the CL reaction were investigated and optimized systematically. Under the optimum conditions, it was found that the CL intensity is proportional to the concentration of gatifloxacin in the range of 1.12 × 10−11–4.40 × 10−9 g mL−1 with a co-relation coefficient of 0.9994. The limit of detection was found to be 4.87 × 10−12 g mL−1 and the relative standard deviation (RSD, n = 7) was 1.8% for 4 × 10−8 g mL−1 of GFLX. The proposed method offers higher sensitivity, wide linear range and better stability without requiring sophisticated instrumentation. Thus, the proposed method has been successfully applied to the determination of gatifloxacin in pharmaceuticals, serum and human urine.
KeywordsChemiluminescence Gatifloxacin Sodium hyposulphite Sodium dodecyl benzene sulphonate Flow-injection
This research was supported by Kyungpook National University Research Fund, 2009.
- 4.Wei L, Chao W, Hong J (2009) Determination of gatifloxacin by spectrophotometric method with malachite green. Huaxue Shiji 31:191–193Google Scholar
- 5.Kshirsagar SJ, Chopade VV, Dhaneshwar SR (2008) A sensitive first and second derivative spectrophotometric method and validation for determination of gatifloxacin in bulk and pharmaceutical dosage forms. BioScan 3(2):147–150Google Scholar
- 15.Nguyen HA, Grellet J, Ba BB, Quentin C, Saux MC (2004) Simultaneous determination of levofloxacin, gatifloxacin and moxifloxacin in serum by liquid chromatography with column switching. J Chromatogr B 810(1):77–83Google Scholar
- 18.Bin Z, Shou-jun J, Chang-qin HU (2002) Determination of gatifloxacin by high performance capillary electrophoresis. Chin J Pharm Anal 22:397–400Google Scholar
- 21.Sun H, Li L, Chen X (2008) Determination of gatifloxacin in injection solutions and urine by europium-sensitized chemiluminescence. Asian J Pharm Sci 3(4):168–174Google Scholar
- 23.Townshend A, Ruengsitagoon W, Thongpoon C, Liawruangrath S (2005) Flow injection chemiluminescence determination of tetracycline. Anal Chim Acta 541(1–2):105–111Google Scholar
- 24.Anastos N, Barnet NW, Lewis SW, Gathergood N, Scammells PJ, Sims DN (2005) Determination of psilocin and psilocybin using flow injection analysis with acidic potassium permanganate and tris(2, 2′-bipyridyl)ruthenium(II) chemiluminescence detection respectively. Talanta 67(2):354–359PubMedCrossRefGoogle Scholar
- 27.Kenner CT, Bush KW (1979) Quantitative Analysis. Macmillan, New York, p 375Google Scholar