Journal of Fluorescence

, Volume 21, Issue 4, pp 1539–1545 | Cite as

Ultrasensitive Study of Gatifloxacin Based on Its Enhancing Effect on the Cerium (IV)-Sodium Hyposulfite Chemiluminescence Reaction in a Micellar Medium

  • Mohammad Kamruzzaman
  • Al-Mahmnur Alam
  • Taslima Ferdous
  • Sang Hak Lee
  • Young Ho Kim
  • Sung Hong Kim
Original Paper


A sensitive and rapid flow-injection chemiluminescence (CL) method has been developed for the determination of gatifloxacin in pharmaceutical preparations and biological samples. The method is based on the enhancing effect of gatifloxacin on CL emission generated by the interaction of Ce (IV) in sulphuric acid and sodium hyposulphite (Na2S2O4) sensitized by sodium dodecyl benzene sulfonate (SDBS). Strong CL emission was observed when gatifloxacin was injected into the Ce (IV) in sulphuric acid and Na2S2O4 solution incorporated with SDBS in a flow-cell. Several experimental parameters affecting the CL reaction were investigated and optimized systematically. Under the optimum conditions, it was found that the CL intensity is proportional to the concentration of gatifloxacin in the range of 1.12 × 10−11–4.40 × 10−9 g mL−1 with a co-relation coefficient of 0.9994. The limit of detection was found to be 4.87 × 10−12 g mL−1 and the relative standard deviation (RSD, n = 7) was 1.8% for 4 × 10−8 g mL−1 of GFLX. The proposed method offers higher sensitivity, wide linear range and better stability without requiring sophisticated instrumentation. Thus, the proposed method has been successfully applied to the determination of gatifloxacin in pharmaceuticals, serum and human urine.


Chemiluminescence Gatifloxacin Sodium hyposulphite Sodium dodecyl benzene sulphonate Flow-injection 



This research was supported by Kyungpook National University Research Fund, 2009.

Supplementary material

10895_2011_842_MOESM1_ESM.doc (180 kb)
ESM 1 (DOC 180 kb)


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Mohammad Kamruzzaman
    • 1
  • Al-Mahmnur Alam
    • 1
  • Taslima Ferdous
    • 1
  • Sang Hak Lee
    • 1
    • 3
  • Young Ho Kim
    • 2
  • Sung Hong Kim
    • 3
  1. 1.Department of ChemistryKyungpook National UniversityDaeguSouth Korea
  2. 2.Research Institute of Advanced Energy TechnologyKyungpook National UniversityDaeguSouth Korea
  3. 3.Korea Basic Science Institute Daegu CenterDaeguSouth Korea

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