Journal of Fluorescence

, Volume 21, Issue 2, pp 739–745 | Cite as

Spectrofluorimetric Assessment of Metoclopramide Hydrochloride Using Terbium Doped in PMMA Matrix Optical Sensor

  • M. S. Attia
  • A. M. Othman
  • E. Elraghi
  • Hassan Y. Aboul-Enein
Original Paper


A new, simple and accurate spectrofluorimetric method for the determination of metoclopramide hydrochloride was developed. The metoclopramide hydrochloride can remarkably enhance the luminescence intensity of the Tb3+ ion doped in PMMA matrix at λex = 360 nm in methanol at pH 6.9. The intensity of the emission band at 545 nm of Tb3+ ion doped in PMMA matrix is increased due to the energy transfer from metoclopramide hydrochloride to Tb3+ in the excited stated. The effect of different parameters, e.g., pH, temperature, Tb3+ concentration, foreign ions that control the fluorescence intensity of the produced ion associate was critically investigated. The calibration curve of the emission intensity at 545 nm shows linear response of metoclopramide over a concentration range of 5 × 10−5–5.0 × 10−8 M with detection limit of 8.7 × 10−10 M. The method was used successfully for the determination of metoclopramide in pharmaceutical preparations and human serum. The average recovery of 99.48% with standard deviation of 0.32% and 96.98% with standard deviation of 0.4%, of pharmaceutical preparations and human serum respectively, were obtained which compared will with the results obtained from standard LC method of average recovery 99.04% and standard deviation of 0.6% and average recovery of 98.19% with standard deviation of 0.6% of pharmaceutical preparations and human serum, respectively.


Metoclopramide hydrochloride Terbium Optical sensor Energy transfer Luminescence Intensity PMMA 


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Department of Chemistry, Faculty of ScienceAin Shams UniversityCairoEgypt
  2. 2.Environmental Biotechnology, Genetic Engineering and Biotechnology InstituteMenoufia UniversitySadatEgypt
  3. 3.Pharmaceutical and Medicinal Chemistry DepartmentNational Research CentreCairoEgypt

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