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Journal of Fluorescence

, Volume 20, Issue 2, pp 625–629 | Cite as

Fluorescence Quenching to Study Protein-ligand Binding: Common Errors

  • Marco van de Weert
Short Communication

Abstract

A number of recent articles, amongst others several published in the Journal of Fluorescence, use inappropriate fluorescence methodology to determine ligand binding characteristics to (mostly) proteins. In this Letter, several common pitfalls are discussed in relation to two recent publications in the Journal of Fluorescence (Wang et al. (2009) 19:801–808; Ding et al. (2009) 19:783–791). The Author hopes that this contribution helps to prevent a further spread of the incorrect methodology, and results in a reappraisal of those articles already published using similar methodology.

Keywords

Fluorescence quenching FRET Ligand binding Inner-filter effect 

References

  1. 1.
    Wang Z, Tan X, Chen W, Yue Q, Song Z (2009) Study of the binding behavior of lysozyme with cephalosporin analogues by fluorescence spectroscopy. J Fluoresc 19:801–808CrossRefPubMedGoogle Scholar
  2. 2.
    Ding F, Liu W, Liu F, Li Z-Y, Sun Y (2009) A study of the interaction between malachite green and lysozyme by steady-state fluorescence. J Fluoresc 19:783–791CrossRefPubMedGoogle Scholar
  3. 3.
    Gök E, Öztürk C, Akbay N (2008) Interaction of thyroxine with 7 hydroxycoumarin: a fluorescence quenching study. J Fluoresc 18:781–785CrossRefPubMedGoogle Scholar
  4. 4.
    He Y, Wang Y, Tang L, Liu H, Chen W, Zheng Z, Zou G (2008) Binding of puerarin to human serum albumin: a spectroscopic analysis and molecular docking. J Fluoresc 18:433–442CrossRefPubMedGoogle Scholar
  5. 5.
    Lu Z, Zhang Y, Liu H, Yuan J, Zheng Z, Zou G (2007) Transport of a cancer chemopreventive polyphenol, resveratrol: interaction with serum albumin and hemoglobin. J Fluoresc 17:580–587CrossRefPubMedGoogle Scholar
  6. 6.
    Ran D, Wu X, Zheng J, Yang J, Zhou H, Zhang M, Tang Y (2007) Study on the interaction between florasulam and bovine serum albumin. J Fluoresc 17:721–726CrossRefPubMedGoogle Scholar
  7. 7.
    Xiang G, Tong C, Lin H (2007) Nitroaniline isomers interaction with bovine serum albumin and toxicological implications. J Fluoresc 17:512–521CrossRefPubMedGoogle Scholar
  8. 8.
    Xiao JB, Chen XQ, Jiang XY, Hilczer M, Tachiya M (2008) Probing the interaction of trans-resveratrol with bovine serum albumin: a fluorescence quenching study with Tachiya model. J Fluoresc 18:671–678CrossRefPubMedGoogle Scholar
  9. 9.
    Yue Q, Niu L, Li X, Shao X, Xie X, Song Z (2008) Study on the interaction mechanism of lysozyme and bromophenol blue by fluorescence spectroscopy. J Fluoresc 18:11–15CrossRefPubMedGoogle Scholar
  10. 10.
    Zhang H-G, Huang X, Mei P, Li K-H, Yan C-N (2006) Studies on the interaction of tricyclazole with b-cyclodextrin and human serum albumin by spectroscopy. J Fluoresc 16:287–294CrossRefPubMedGoogle Scholar
  11. 11.
    Zhang Y-Z, Zhou B, Liu Y-X, Zhou C-X, Ding X-L, Liu Y (2008) Fluorescence study on the interaction of bovine serum albumin with p-aminoazobenzene. J Fluoresc 18:109–118CrossRefPubMedGoogle Scholar
  12. 12.
    Zhang Y, Dong L, Li Y, Li J, Chen X (2008) Characterization of interaction between bergenin and human serum albumin in membrane mimetic environments. J Fluoresc 18:661–670CrossRefPubMedGoogle Scholar
  13. 13.
    Zhang H-X, Huang X, Zhang M (2008) Thermodynamic studies on the interaction of dioxopromethazine to β-cyclodextrin and bovine serum albumin. J Fluoresc 18:753–760CrossRefPubMedGoogle Scholar
  14. 14.
    Akbay N, Seferoglu Z, Gök E (2009) Fluorescence interaction and determination of calf thymus DNA with two ethidium derivatives. J Fluoresc, doi: 10.1007/s10895-009-0504-9
  15. 15.
    Epps DE, Raub TJ, Kézdy FJ (1995) A general, wide-range spectrofluorimetric method for measuring the site-specific affinities of drugs toward human serum albumin. Anal Biochem 227:342–350CrossRefPubMedGoogle Scholar
  16. 16.
    Bi S, Ding L, Tian Y, Song D, Zhou X, Liu X, Zhang H (2004) Investigation of the interaction between flavonoids and human serum albumin. J Mol Struct 703:37–45CrossRefGoogle Scholar
  17. 17.
    Lakowicz JR (1999) Principles of fluorescence spectroscopy. Kluwer Academic/Plenum, New YorkGoogle Scholar
  18. 18.
    Chacon P, Moran F, Diaz JF, Pantos E, Andreu JM (1998) Low-resolution structures of proteins in solution retrieved from x-ray scattering with a genetic algorithm. Biophys J 74:2760–2775CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Department of Pharmaceutics and Analytical Chemistry, Faculty of Pharmaceutical SciencesUniversity of CopenhagenCopenhagenDenmark

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