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Fluorescent Probe ABM and Estimation of Immune State in Patients with Different Pathologies (Review Article)

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Abstract

The fluorescent probe ABM (3-aminobenzanthrone derivative) one of the fluorescent probes synthesized in Riga Technical University proved to be an excellent, independent model for studying cell membranes. In our work we have investigated the possibility of using the fluorescent probe ABM for detection of immune state in patients with different pathologies. There is a strong correlation among all studied ABM spectral parameters, immunological characteristics, clinical and laboratory investigations of the all observed patients groups. The obtained results suggest that ABM spectral parameters in cell suspension reflect the alterations of the cellular mechanisms of immunity. Therefore fluorescent method could be used as preliminary screening test in immune diagnostics instead of more expensive, time consuming methods (subset detection, radioisotope method etc.) used as routine in clinics. Spectral parameters of ABM reflect a wide range of interrelated (interdependent) characteristics of cells (physico-chemical state and microviscosity of membrane, proliferating and lipid metabolic activity of cells, distribution of cells among subsets). The observed change of the studied parameters reflects alterations of the cellular mechanisms of immunity which is a main focus for its application as preliminary screening test in immune diagnostics. The fluorescence based method is sensitive, less expensive and time consuming, technically simple and convenient.

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Authors and Affiliations

  1. Daugavpils University, Daugavpils, Latvia

    Inta Kalnina & Elena Kirilova

  2. Biomedical Research and Study Centre, University of Latvia, Riga, Latvia

    Ruta Bruvere & Natalija Gabruseva

  3. Institute of Occupational and Environmental Health, Riga Stradins University, Riga, Latvia

    Tija Zvagule

  4. Latvian Centre of Oncology, Riga, Latvia

    Laura Klimkane

  5. Riga Technical University, Riga, Latvia

    Imants Meirovics

  6. University of Latvia, Riga, Latvia

    Gunta Kizane

Authors
  1. Inta Kalnina
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  2. Ruta Bruvere
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  3. Tija Zvagule
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  4. Natalija Gabruseva
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  5. Laura Klimkane
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  6. Elena Kirilova
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  7. Imants Meirovics
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  8. Gunta Kizane
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Correspondence to Inta Kalnina.

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Kalnina, I., Bruvere, R., Zvagule, T. et al. Fluorescent Probe ABM and Estimation of Immune State in Patients with Different Pathologies (Review Article). J Fluoresc 20, 9–17 (2010). https://doi.org/10.1007/s10895-009-0515-6

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  • DOI: https://doi.org/10.1007/s10895-009-0515-6

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