Advertisement

Journal of Fluorescence

, Volume 19, Issue 4, pp 747–753 | Cite as

An Evaluation of a Fluorometric Method for Determining Binding Parameters of Drug–Carrier Complexes Using Mathematical Models Based on Total Drug Concentration

  • Boontarika Chanvorachote
  • Ubonthip Nimmannit
  • Walaisiri Muangsiri
  • Lee Kirsch
Original Paper

Abstract

A fluorescence method for determining the mode of binding and estimating binding parameters in a model drug-carrier complex was developed using the lipopeptide antibiotic daptomycin and polyamidoamine (PAMAM) dendrimer. Mathematical simulations of model equations describing fluorescence changes induced by antibiotic–carrier binding in terms of total drug concentration were used to evaluate the sensitivity of parameter variation on binding isotherms for both one- and two-site binding models. Nonlinear regression analysis was used to estimate binding parameters and to identify pH-dependent binding models.

Keywords

Fluorescence methods Drug binding Dissociation constant Capacity constant and Drugcarrier complexes 

References

  1. 1.
    Conners A (1987) Binding constants, the measurement of molecular complex stability. Wiley, New YorkGoogle Scholar
  2. 2.
    Cryan S-A (2005) Carrier-based strategies for targeting protein and peptide drugs to the lungs. AAPS J 07(01):E20–E41 doi: 10.1208/aapsj070104 CrossRefGoogle Scholar
  3. 3.
    Epps E, Raub TJ, Caiolfa V, Chiari A, Zamai M (1999) Determination of the affinity of drugs toward serum albumin by measurement of the quenching of the intrinsic tryptophan fluorescence of the protein. J Pharm Pharmacol 51(1):41–48 doi: 10.1211/0022357991772079 PubMedCrossRefGoogle Scholar
  4. 4.
    Jung D, Rozek A, Okon M, Hancock REW (2004) Structural transitions as determinants of the action of the calcium-dependent antibiotic daptomycin. Chem Biol 11:949–957 doi: 10.1016/j.chembiol.2004.04.020 PubMedCrossRefGoogle Scholar
  5. 5.
    Klajnert B, Pastucha A, Shcharbin D, Bryszewska M (2007) Binding properties of polyamidoamine dendrimers. J Appl Polym Sci 103:2036–2040 doi: 10.1002/app.25279 CrossRefGoogle Scholar
  6. 6.
    Lakey JH, Ptak M (1988) Fluorescence indicates a calcium-dependent interaction between the lipopeptide antibiotic LY146032 and phospholipid membranes. Biochemistry 27:4639–4645 doi: 10.1021/bi00413a009 PubMedCrossRefGoogle Scholar
  7. 7.
    Lakey JH, Maget-Dana R, Ptak M (1989) The lipopeptide antibiotic A21978C has a specific interaction with DMPC only in the presence of calcium ions. Biochim Biophys Acta 985:60–66 doi: 10.1016/0005-2736(89)90104-1 PubMedCrossRefGoogle Scholar
  8. 8.
    Lakowicz R (2006) Principles of fluorescence spectroscopy, 3rd edn. Springer, BerlinCrossRefGoogle Scholar
  9. 9.
    Larsson Å (1997) Regression analysis of simulated radio-ligand equilibrium experiments using seven different mathematical models. J Immunol Methods 206(1–2):135–142PubMedCrossRefGoogle Scholar
  10. 10.
    Liu HL, Liang KH, Xu LL, Tong B (2007) Tang: Supramolecular interaction of ethylenediamine linked beta-cyclodextrin dimer and berberine hydrochloride by spectrofluorimetry and its analytical application. Talanta 74(1):140–145 doi: 10.1016/j.talanta.2007.05.048 PubMedCrossRefGoogle Scholar
  11. 11.
    Muangsiri W, Kirsch LE (2006) The protein-binding and drug release properties of macromolecular conjugates containing daptomycin and dextran. Int J Pharm 315:30–43 doi: 10.1016/j.ijpharm.2006.02.016 PubMedCrossRefGoogle Scholar
  12. 12.
    Okabe N, Hashizume N (1994) Drug binding properties of glycosylated human serum albumin as measured by fluorescence and circular dichroism. Biol Pharm Bull 17(1):16–21PubMedGoogle Scholar
  13. 13.
    Parikh HH, Mcelwain K, Balasubramanian V, Leung W, Wong D, Morrise ME, Ramanathan M (2000) A rapid spectrofluorimetric technique for determining drug-serum protein binding suitable for high-throughput screening. Pharm Res 17(5):632–637 doi: 10.1023/A:1007537520620 PubMedCrossRefGoogle Scholar
  14. 14.
    Qiu J,, Kirsch LE (2007) Evaluation of lipopeptide aggregation (Daptomcyin) using light scattering, fluorescence, and NMR spectroscopy. AAPS J 9:S2Google Scholar
  15. 15.
    Rawat D, Singh S, Saraf S, Saraf S (2006) Nanocarriers: promising vehicle for bioactive drugs. Biol Pharm Bull 29(9):1790–1798 doi: 10.1248/bpb.29.1790 PubMedCrossRefGoogle Scholar
  16. 16.
    Svenson S, Tomalia DA (2005) Dendrimers in biomedical applications—reflections on the field B. Adv Drug Deliv Rev 57:2106–2129 doi: 10.1016/j.addr.2005.09.018 PubMedCrossRefGoogle Scholar
  17. 17.
    Thomas P, Nelson G, Patonay G, Warner IM (1988) Analysis of drug binding sites on human serum albumin using multidimensional fluorescence measurements. Spectrochimica Acta 43B(4–5):651–660Google Scholar
  18. 18.
    Wang YP, Wei YL, Chuan D (2006) Study on the interaction of 3,3-bis(4-hydroxy-1-naphthyl)-phthalide with bovine serum albumin by fluorescence spectroscopy. J Photochem Photobiol Chem 177:6–11 doi: 10.1016/j.jphotochem.2005.04.040 CrossRefGoogle Scholar
  19. 19.
    Wang Z-X, Jiang R-F (1996) A novel two-site binding equation presented in terms of the total ligand concentration. FEBS Lett 392:245–249 doi: 10.1016/0014-5793(96)00818-6 PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Boontarika Chanvorachote
    • 1
  • Ubonthip Nimmannit
    • 1
  • Walaisiri Muangsiri
    • 1
  • Lee Kirsch
    • 2
  1. 1.Faculty of Pharmaceutical ScienceChulalongkorn UniversityBangkokThailand
  2. 2.Division of Pharmaceutics, College of PharmacyThe University of IowaIowa CityUSA

Personalised recommendations