An Evaluation of a Fluorometric Method for Determining Binding Parameters of Drug–Carrier Complexes Using Mathematical Models Based on Total Drug Concentration
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A fluorescence method for determining the mode of binding and estimating binding parameters in a model drug-carrier complex was developed using the lipopeptide antibiotic daptomycin and polyamidoamine (PAMAM) dendrimer. Mathematical simulations of model equations describing fluorescence changes induced by antibiotic–carrier binding in terms of total drug concentration were used to evaluate the sensitivity of parameter variation on binding isotherms for both one- and two-site binding models. Nonlinear regression analysis was used to estimate binding parameters and to identify pH-dependent binding models.
KeywordsFluorescence methods Drug binding Dissociation constant Capacity constant and Drugcarrier complexes
- 1.Conners A (1987) Binding constants, the measurement of molecular complex stability. Wiley, New YorkGoogle Scholar
- 14.Qiu J,, Kirsch LE (2007) Evaluation of lipopeptide aggregation (Daptomcyin) using light scattering, fluorescence, and NMR spectroscopy. AAPS J 9:S2Google Scholar
- 17.Thomas P, Nelson G, Patonay G, Warner IM (1988) Analysis of drug binding sites on human serum albumin using multidimensional fluorescence measurements. Spectrochimica Acta 43B(4–5):651–660Google Scholar