Versatile Nitro-Fluorophore as Highly Effective Sensor for Hypoxic Tumor Cells: Design, Imaging and Evaluation
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Based on the characteristics of a nitro-group, which can be partially or totally reduced to an amino-group in hypoxic cells, two series of hypoxic sensors containing 7H-Benzimidazole [2,1-a]benz[de]isoquinolin-7-one cores with nitro groups in different positions were designed, synthesized and evaluated. The target compounds exhibited significantly different fluorescence characteristics (fluorescence enhancement or quenching). The strong fluorescences of some partially reduced compounds could be explained through the examination of their infrared spectra, which showed a restriction of the nitro-group vibration. These compounds exhibited significant hypoxic–oxic fluorescence differences not only in numerical values but also in their fluorescent imaging properties as reported for the first time.
KeywordsNitro-fluorophore Sensor Hypoxic Tumor cell
Financial support by National Natural Science Foundation of China and Program of Shanghai Subject Chief Scientist and the National Key Project for Basic Research (2003CB114400) is greatly appreciated. We also appreciated Dr. Ingo for the improvement of English expression.
- 13.Krasovitskii BM, Bolotin BM Organic Luminescent Materials. Translated by Vopian V.G., Weinheim:VCH, 1988Google Scholar
- 14.Shershukov VM, Efimova SL, Malyukin YV, Skripkina VT, Doroshenko AO, Ponomarev OA (1995) Structure and absorption-luminescence spectral properties of 1,8-naphthoylene -1¢,2¢-benzimidazole derivatives containing substituents of different electronic nature. Khimiya. Geterotsiklicheskikh. Soedinenii (5):633–639 (English Abstract)Google Scholar