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Journal of Fluorescence

, Volume 18, Issue 2, pp 573–579 | Cite as

Solid Substrate–Room Temperature Phosphorimetry for the Determination of Trace Terbutaline Sulfate Based on Its Inhibition Oxidation of Rhodamine 6G by Sodium Periodate

  • Jia-Ming Liu
  • Fei Gao
  • Wen-Yan Gao
  • Li-Qing Zeng
  • Xiao-Mei Huang
  • Zhi-Ming Li
  • Xiu-Chai Huang
  • Wei-Nv Lin
  • Fang-Mei Wang
  • Chang-Ling Nie
Original Paper

Abstract

When 1.00 mol l−1 I is used as ion perturber, rhodamine 6G (Rh 6G) can emit strong and stable room temperature phosphorescence (RTP) on filter paper substrate in KHC8H4O4–HCl buffer solution (pH = 3.50), heated at 70 °C for 10 min. NaIO4 can oxidize Rh 6G, which makes the RTP signal quench. Terbutaline sulfate (TBS) can inhibit NaIO4 from oxidizing Rh 6G, which makes the RTP signal of Rh 6G enhance sharply. The content of TBS is linear correlation to ΔIp of the system. Based on the facts above, a new inhibition solid substrate-room temperature phosphorimetry (SS-RTP) for the determination of trace TBS has been established. The linear range of this method is 0.0104–2.08 pg spot−1 (corresponding concentration: 0.026–5.2 ng ml−1, with a sample volume of 0.4 μl) with a detection limit (L.D.) of 2.6 fg spot−1 (corresponding concentration: 6.5 × 10−12 g ml−1), and the regression equation of working curve is ΔIp = 2.040 + 54.54 mTBS (pg spot−1), n = 6, correlation coefficient is 0.9994. For the samples containing 0.0104 pg spot−1 and 2.08 pg spot−1 TBS, the relative standard deviation (RSD) are 3.8% and 2.3% (n = 8), respectively, indicating good precision. This method has been applied to determination of trace TBS in the practical samples with satisfactory results. The reaction mechanism of NaIO4 oxidizing Rh 6G to inhibit SS-RTP for the determination of trace TBS is also discussed.

Keywords

Terbutaline sulfate Rhodamine 6G Inhibition solid substrate–room temperature phosphorimetry 

Notes

Acknowledgements

This work was supported by Fujian Province Science Foundation (Grant No. 2006J0386, 2006J0269, C0510028, D0510027).

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Jia-Ming Liu
    • 1
  • Fei Gao
    • 1
  • Wen-Yan Gao
    • 1
  • Li-Qing Zeng
    • 1
    • 2
  • Xiao-Mei Huang
    • 1
    • 2
  • Zhi-Ming Li
    • 2
  • Xiu-Chai Huang
    • 1
  • Wei-Nv Lin
    • 1
  • Fang-Mei Wang
    • 1
  • Chang-Ling Nie
    • 3
  1. 1.Department of ChemistryZhangzhou Normal CollegeZhangzhouPeople’s Republic of China
  2. 2.Department of Food and Biological EngineeringZhangzhou Institute of TechnologyZhangzhouPeople’s Republic of China
  3. 3.The Night Middle School, SanmingSanmingPeople’s Republic of China

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