Is Phosphorescence Lifetime an Indicator of Angiogenesis in Cortical Sarcoma?
In this paper, the oxygen dependent phosphorescence quenching method is proposed to study the correlation between the phosphorescence lifetime and microvessel density in tumors. In the implementation, the S180 transplanted tumor in the mouse is used for the collection of the time-resolved phosphorescence, the tumor microvessel density is measured by immunohistochemical examination of FVIII, the correlation between microvessel density and phosphorescence lifetime is analyzed by multiple regression method. The results show the phosphorescence decay constant measured in tumors is enlarged in the tumor progression. Furthermore, the relative total microvessel area is positively correlative with the phosphorescence lifetime, which is estimated by a two dimension regression equation. It is concluded phosphorescence lifetime is a valuable indicator of angiogenesis during the tumor development.
KeywordsAngiogenesis Microvessel density Phosphorescence quenching Pd-TCPP Phosphorescence lifetime
The work was supported by Shenzhen Science and Technology Program (No.200519).
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