Is Phosphorescence Lifetime an Indicator of Angiogenesis in Cortical Sarcoma?
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In this paper, the oxygen dependent phosphorescence quenching method is proposed to study the correlation between the phosphorescence lifetime and microvessel density in tumors. In the implementation, the S180 transplanted tumor in the mouse is used for the collection of the time-resolved phosphorescence, the tumor microvessel density is measured by immunohistochemical examination of FVIII, the correlation between microvessel density and phosphorescence lifetime is analyzed by multiple regression method. The results show the phosphorescence decay constant measured in tumors is enlarged in the tumor progression. Furthermore, the relative total microvessel area is positively correlative with the phosphorescence lifetime, which is estimated by a two dimension regression equation. It is concluded phosphorescence lifetime is a valuable indicator of angiogenesis during the tumor development.
KeywordsAngiogenesis Microvessel density Phosphorescence quenching Pd-TCPP Phosphorescence lifetime
The work was supported by Shenzhen Science and Technology Program (No.200519).
- 15.Bottiroli G, Croce AC, Locatelli D, Nano R, Giombelli E, Messina A, Benericetti E (1998) Brain tissue autofluorescence: an aid for intraoperative delineation of tumor resection margins, Cancer detection. Prevent 22(4):330–339Google Scholar
- 16.Lakowicz J (1983) Principles of fluorescence spectroscopy. Plenum, New YorkGoogle Scholar
- 23.Lee SK, Shin YB, Pyo HB, Park SH, Ogura SI, Okura I (2001) Measurement of oxygen concentrations in tumor cells by the phosphorescence quenching method. Bull Korean Chem Soc 22(3):259–260Google Scholar
- 31.Braun RD, Lanzen JL, Snyder SA, Dewhirst MW (2001) Comparison of tumor and normal tissue oxygen tension measurements using OxyLite or microelectrodes in rodents. Am J Physiol 280:H2533–H2544Google Scholar