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Journal of Fluorescence

, Volume 17, Issue 3, pp 293–300 | Cite as

Fluorimetric Determination of Some Sulfur Containing Compounds Through Complex Formation with Terbium (Tb+3) and Uranium (U+3)

  • Elham Anwer Taha
  • Nagiba Yehya Hassan
  • Fahima Abdel Aal
  • Laila El-Sayed Abdel Fattah
Original Paper

Abstract

Two simple, sensitive and specific fluorimetric methods have been developed for the determination of some sulphur containing compounds namely, Acetylcysteine (Ac), Carbocisteine (Cc) and Thioctic acid (Th) using terbium Tb+3 and uranium U+3 ions as fluorescent probes. The proposed methods involve the formation of a ternary complex with Tb+3 in presence of Tris-buffer method (I) and a binary complex with aqueous uranyl acetate solution method (II). The fluorescence quenching of Tb+3 at 510, 488 and 540 nm (λex 250, 241 and 268 nm) and of uranyl acetate at 512 nm (λex 240 nm) due to the complex formation was quantitatively measured for Ac, Cc and Th, respectively. The reaction conditions and the fluorescence spectral properties of the complexes have been investigated. Under the described conditions, the proposed methods were applicable over the concentration range (0.2–2.5 μg ml−1), (1–4 μg ml−1) and (0.5–3.5 μg ml−1) with mean percentage recoveries 99.74±0.36, 99.70±0.52 and 99.43±0.23 for method (I) and (0.5–6 μg ml−1), (0.5–5 μg ml−1), and (1–6 μg ml−1) with mean percentage recoveries 99.38±0.20, 99.82±0.28 and 99.93±0.32 for method (II), for the three cited drugs, respectively. The proposed methods were successfully applied for the determination of the studied compounds in bulk powders and in pharmaceutical formulations, as well as in presence of their related substances. The results obtained were found to be in agree statistically with those obtained by official and reported ones. The two methods were validated according to USP guidelines and also assessed by applying the standard addition technique.

Keywords

Fluorimetric determination Acetylcysteine Carbocisteine Thioctic acid Terbium chloride Uranyl acetate 

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Elham Anwer Taha
    • 1
  • Nagiba Yehya Hassan
    • 2
  • Fahima Abdel Aal
    • 1
  • Laila El-Sayed Abdel Fattah
    • 2
  1. 1.National Organization for Drug Control and Research (NODCAR), 6 Abu Hazem. PyramidsGizaEgypt
  2. 2.Analytical Chemistry Department, Faculty of pharmacyCairo UniversityCairoEgypt

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