Journal of Fluorescence

, Volume 16, Issue 6, pp 831–837 | Cite as

Sensitive Determination of Norfloxacin by the Fluorescence Probe of Terbium (III)- Sodium Dodecylbenzene Sulfonate and Its Luminescence Mechanism

Original Paper


The fluorescence system of the norfloxacin-Tb3+- sodium dodecylbenzene sulfonate (SDBS) was investigated in this paper. The experiments indicated that the fluorescence intensity of the Tb3+-SDBS was greatly enhanced by the norfloxacin. On the basis of the above findings, a sensitive fluorimetric method for determining the norfloxacin was established. The fluorescence intensity was measured by a 1-cm quartz cell with the excitation wavelength of 290 nm and the emission wavelength of 545 nm. The enhanced fluorescence intensity of the system (Δ F) showed a good linear relationship with the concentration of norfloxacin in the range of 5.0×10−9 mol L−1–2.0×10−6 mol L−1, its correlation coefficient was 0.9991 and the detection limit (S/N=3) was 1.2×10−9 mol L−1. The presented method was used to determine the norfloxacin in real pharmaceutical samples. The luminescence mechanism was also discussed in detail. In the fluorescence system of the norfloxacin-Tb3+-SDBS, the SDBS not only acted as the surfactant, but also acted as the energy donor.


Terbium(III) Norfloxacin Spectrofluorimetry Enhancement effect Determination 



This work was supported by the National Natural Science Foundation of China (No. 20577039) and Zhejiang Provincial Natural Science Foundation of China (No. M203088).


  1. 1.
    Rahman N, Ahmad Y, Azmi SNH (2004) Kinetic spectrophotometric method for the determination of norfloxacin in pharmaceutical formulations. Eur J Pharm Biopharm 57(2):359–367PubMedCrossRefGoogle Scholar
  2. 2.
    Zhang ZQ, Jiang YC (2001) Flow injection flame atomic absorption spectrometry for the indirect analysis of norfloxacin. Atom Spectrosc 22(6):429–432Google Scholar
  3. 3.
    Kowalski C, Rolinski Z, Slawik T, Glod BK (2005) Determination of norfloxacin in chicken tissues by HPLC with fluorescence detection. J Liq Chromatogr R T 28(1):121–135CrossRefGoogle Scholar
  4. 4.
    Espinosa-Mansilla A, de la Pena AM, Gomez DG, Salinas F (2005) HPLC determination of enoxacin, ciprofloxacin, norfloxacin and ofloxacin with photoinduced fluorimetric(PIF) detection and multiemission scanning - Application to urine and serum. J Chromatogr B 822(1–2):185–193CrossRefGoogle Scholar
  5. 5.
    Du JX, Li YH, Lu JR (2005) Chemiluminescence determination of fluoroquinolone antibiotics using a soluble manganese (IV)-sulphite system. Luminescence 20(1):30–35PubMedCrossRefGoogle Scholar
  6. 6.
    Hu M, Hu CQ, Liu WY (2004) Determination of drug reference substance content by H-1-nuclear magnetic resonance spectroscopy. Chinese J Anal Chem 32(4):451–455Google Scholar
  7. 7.
    Liu ZH, Huang ZY, Cai RX (2000) Study of the fluorescence characteristics of norfloxacin in reversed micelles and application in analysis. Analyst 125(8):1477–1481PubMedCrossRefGoogle Scholar
  8. 8.
    Du LM, Cao XM, Wu HY (2003) Direct determination of enoxacin in human plasma by micellar enhancing effect-synchronous fluorimetry. Chinese J Anal Chem 31:1330–1332Google Scholar
  9. 9.
    Han YX, Wu X, Yang JH, Sun SN (2005) The fluorescence characteristic of the yttrium-norfloxacin system and its analytical application. J Pharm Biomed Anal 38(3):528–531PubMedCrossRefGoogle Scholar
  10. 10.
    Tong CL, Zhu Y, Yang JH (2000) Terbium ion fluorescence probe for the determination of epinephrine. Chinese J Anal Chem 28(3):293–295Google Scholar
  11. 11.
    Tong SL, Xiang GH, Chen PP (2004) Fluorescence reaction between terbium(III) ion and norepinephrine and its analytical application. Spectrosc Spect Anal 24(12):1612–1614Google Scholar
  12. 12.
    Tong CL, Hu Z, Liu WP (2005) Sensitive determination of DNA based on the interaction between norfloxacin-Tb3+ complex and DNA. J Agr Food Chem 53(16):6207–6212CrossRefGoogle Scholar
  13. 13.
    Martinez EJL, Reyes JFG, Barrales PO, Diaz AM (2005) Terbium-sensitized luminescence optosensor for the determination of norfloxacin in biological fluids. Anal Chim Acta 532(2):159–164CrossRefGoogle Scholar
  14. 14.
    Zhao HC, Zhang HL, Zhang YA (1998) A study of the fluorescence system of norfloxacin-terbium and the determination of norfloxacin. Chinese J Anal Lab 17(3):27–29 (in Chinese)Google Scholar
  15. 15.
    Tong CL, Zhu Y, Liu WP (2001). Study on the co-luminescence system of Dy-Gd-1,6-bis(1′-phenyl-3′-methyl-5′-pyrazol-4′-one) hexanedione-cetyltrimethyl lammonium bromide and its analytical application. Analyst 126(7):1168–1171PubMedCrossRefGoogle Scholar
  16. 16.
    Huang ZY, Cai RX, Zhang K, Huang HP, Zeng YE (1997) Micelle enhanced spectrofluorimetric determination of norfloxacin using terbium as fluorescence probe. Anal Lett 30(8):1531–1539Google Scholar
  17. 17.
    Shah SS, Naeem K, Shah SWH, Laghari GM (2000) Differential absorbance measurements of amphiphilic hemicyanine dyes, solubilization study in anionic surfactant. Colloid Surface A 168(1):77–85CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  1. 1.Institute of Environmental Science, Ministry of Education Key Laboratory of Environmental Remediation and Ecological HealthZhejiang UniversityHangzhouChina

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