Journal of Fluorescence

, Volume 16, Issue 1, pp 53–59 | Cite as

The Sensitive Fluorimetric Method for the Determination of Curcumin Using the Enhancement of Mixed Micelle

  • Feng Wang
  • Xia Wu
  • Fei Wang
  • Shufang Liu
  • Zhen Jia
  • Jinghe Yang

Curcumin (C21H20O6) is a natural antioxidant, which is considered to be a very useful compound in health matters, and is employed in the treatment of cardiovascular and arthritic illnesses. It is found that the fluorescence of curcumin is greatly enhanced by mixed micelle of sodium dodecyl benzene sulfonate (SDBS) and cetyltrimethylammonium bromide (CTAB) surfactants. Based on this, a sensitive fluorimetric method for the determination of curcumin in aqueous solution is proposed. In the HOAc–NaOAc buffer, the fluorescence intensity of curcumin is proportional to the concentration of curcumin in the range of 0.00020–0.74 μg/mL and the detection limit is 0.017 ng/mL. The synthetic and actual samples are satisfactorily determined. In addition, the interaction mechanism is also studied.


Fluorescence Curcumin Anionic-cationic surfactant 





Sodium Dodecyl Benzene Sulfonate


Cetyltrimethylammonium bromide


Critical micelle concentration.



The Natural Science Foundations of China (20575035) and Shandong Province, (Y2003B02) and a Visiting Scholar Foundation of the Key Laboratory at Shandong University supported this work.


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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Feng Wang
    • 1
    • 2
  • Xia Wu
    • 1
  • Fei Wang
    • 1
  • Shufang Liu
    • 1
  • Zhen Jia
    • 1
  • Jinghe Yang
    • 1
  1. 1.Key Laboratory of Colloid and Interface Chemistry, School of Chemistry and Chemical EngineeringShandong University, Ministry of EducationJinanP. R. China.
  2. 2.Department of ChemistryZaozhuang UniversityZaozhuangP. R. China.

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