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Projections of Olfactory Bulbs and Non-Olfactory Telencephalic Structures in Amygdaloid Complex of the Turtle Testudo horsfieldi: A Study Using Anterograde Tracer Technique

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Abstract

To confirm the discrete character of projections of telencephalic olfactory and non-olfactory structures to the amygdaloid complex (AC) in the terrestrial turtle Testudo horsfieldi, a study was performed by the method of anterograde axonal transport of tracers (HRP, BDA). After a massive injection of the tracers into the main and accessory olfactory bulb, a dense accumulation of labeled fibers and terminals was found in ventral part of AC in the neuropil zones of nbam (J) and ncoam and very scanty—in nmam and ncam. After a massive injection of the tracers into non-olfactory telencephalic structures including dorsal cortex, pallidal enlargement, and ADVR, a very dense terminal field was observed in the dorsal AC part and a less dense one, with predominance of labeled fibers, in its ventral part. Local administration of the tracers separately into the dorsolateral (visual area) and the ventromedial (auditory-somatic area) parts of the ADVR allowed revealing discrete projections, respectively, to the laterocentral and mediocentral areas of the dorsal AC part with a relative overlapping in the central AC area. In all experiments, retrogradely labeled neurons in AC were also observed in zones of the corresponding bulbar and rostrotelencephalic projections. Thus, it has been shown that in the turtle AC there exist not only separation of direct olfactory and non-olfactory projections, but also discrete projections of different sensory areas of ADVR. Reciprocity of these connections is also confirmed. Organization of afferent olfactory and non-olfactory telencephalic connections in AC is similar in reptiles and in mammals.

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Abbreviations

AC:

Amygdaloid complex

ADVR:

anterior part of the dorsal ventricular ridge

BDA:

biotinylated dextran-amine

HRP:

horseradish peroxidase

OB:

olfactory bulb

PDVR:

posterior part of the dorsal ventricular ridge

amd:

amygdala dorsalis

amv:

amygdala ventralis

cxd:

cortex dorsalis

cxh:

cortex hippocampalis

cxp:

cortex pyriformis

lcm:

lamina cellularis mitralis

lg:

lamina glomerularis

lgi:

lamina granularis interna

nbam (J):

n. basalis amygdalaris (by Johnston)

ncam:

n. centralis amygdalaris

ncoam:

n. corticalis amygdalaris

nmam:

n. medialis amygdalaris

ntol:

n. tractus olphactorius lateralis

path:

pallial thickening

sm:

stria medullaris

str:

striatum

tol:

tractus olfactorius lateralis

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  1. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russia

    D. D. Chkheidze & M. G. Belekhova

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  1. D. D. Chkheidze
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__________

Translated from Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, Vol. 41, No. 5, 2005, pp. 450–456.

Original Russian Text Copyright © 2005 by Chkheidze, Belekhova.

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Chkheidze, D.D., Belekhova, M.G. Projections of Olfactory Bulbs and Non-Olfactory Telencephalic Structures in Amygdaloid Complex of the Turtle Testudo horsfieldi: A Study Using Anterograde Tracer Technique. J Evol Biochem Phys 41, 561–569 (2005). https://doi.org/10.1007/s10893-005-0097-0

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