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Journal of Chemical Ecology

, Volume 44, Issue 4, pp 384–396 | Cite as

Distribution of Defensive Metabolites in Nudibranch Molluscs

  • Anne E. Winters
  • Andrew M. White
  • Ariyanti S. Dewi
  • I. Wayan Mudianta
  • Nerida G. Wilson
  • Louise C. Forster
  • Mary J. Garson
  • Karen L. Cheney
Article

Abstract

Many plants and animals store toxic or unpalatable compounds in tissues that are easily encountered by predators during attack. Defensive compounds can be produced de novo, or obtained from dietary sources and stored directly without selection or modification, or can be selectively sequestered or biotransformed. Storage strategies should be optimized to produce effective defence mechanisms but also prevent autotoxicity of the host. Nudibranch molluscs utilize a diverse range of chemical defences, and we investigated the accumulation and distribution of defensive secondary metabolites in body tissues of 19 species of Chromodorididae nudibranchs. We report different patterns of distribution across tissues, where: 1) the mantle had more or different (but structurally related) compounds than the viscera; 2) all compounds in the mantle were also in the viscera; and 3) the mantle had fewer compounds than the viscera. We found no further examples of species that selectively store a single compound, previously reported in Chromodoris species. Consistent with other studies, we found high concentrations of metabolites in mantle rim tissues compared to the viscera. Using bioassays, compounds in the mantle were more toxic than compounds found in the viscera for Glossodoris vespa Rudman, 1990 and Ceratosoma brevicaudatum Abraham, 1876. In G. vespa, compounds in the mantle were also more unpalatable to palaemonid shrimp than compounds found in the viscera. This indicates that these species may modify compounds to increase bioactivity for defensive purposes and/or selectively store more toxic compounds. We highlight clear differences in the storage of sequestered chemical defences, which may have important implications for species to employ effective defences against a range of predators.

Keywords

Terpenes Chemical defences Sequestration, marine molluscs Aposematism Natural products Bioactivity 

Notes

Acknowledgments

We would like to thank Talia Pettigrew and Gloria Miller for help with chemical extractions and Sean Young for help running assays. This work was funded by the Australian Pacific Science Foundation (APSF) (awarded to KLC and MJG) and The University of Queensland Promoting Women Fellowship (awarded to KLC). Anne Winters was supported by an Endeavour Postgraduate Scholarship.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Anne E. Winters
    • 1
  • Andrew M. White
    • 2
    • 3
  • Ariyanti S. Dewi
    • 2
    • 4
  • I. Wayan Mudianta
    • 2
    • 5
  • Nerida G. Wilson
    • 6
    • 7
  • Louise C. Forster
    • 2
  • Mary J. Garson
    • 2
  • Karen L. Cheney
    • 1
  1. 1.School of Biological SciencesThe University of QueenslandBrisbaneAustralia
  2. 2.School of Chemistry and Molecular BiosciencesThe University of QueenslandBrisbaneAustralia
  3. 3.Institute for Molecular BioscienceThe University of QueenslandBrisbaneAustralia
  4. 4.Agency for Marine and Fisheries ResearchMinistry of Marine Affairs and FisheriesJakartaIndonesia
  5. 5.Universitas Pendidikan GaneshaSingarajaIndonesia
  6. 6.Molecular Systematics Unit, Western Australian MuseumWelshpoolAustralia
  7. 7.School of Biological SciencesUniversity of Western AustraliaCrawleyAustralia

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