Journal of Chemical Ecology

, Volume 36, Issue 4, pp 424–431 | Cite as

Regulation of Reproduction in the Primitively Eusocial Wasp Ropalidia marginata: on the Trail of the Queen Pheromone

  • Anindita Bhadra
  • Aniruddha Mitra
  • Sujata A. Deshpande
  • Kannepalli Chandrasekhar
  • Dattatraya G. Naik
  • Abraham Hefetz
  • Raghavendra Gadagkar


Queens and workers are not morphologically differentiated in the primitively eusocial wasp, Ropalidia marginata. Upon removal of the queen, one of the workers becomes extremely aggressive, but immediately drops her aggression if the queen is returned. If the queen is not returned, this hyper-aggressive individual, the potential queen (PQ), will develop her ovaries, lose her hyper-aggression, and become the next colony queen. Because of the non-aggressive nature of the queen, and because the PQ loses her aggression by the time she starts laying eggs, we hypothesized that regulation of worker reproduction in R. marginata is mediated by pheromones rather than by physical aggression. Based on the immediate loss of aggression by the PQ upon return of the queen, we developed a bioassay to test whether the queen’s Dufour’s gland is, at least, one of the sources of the queen pheromone. Macerates of the queen’s Dufour’s gland, but not that of the worker’s Dufour’s gland, mimic the queen in making the PQ decrease her aggression. We also correctly distinguished queens and workers of R. marginata nests by a discriminant function analysis based on the chemical composition of their respective Dufour’s glands.

Key Words

Ropalidia marginata Queen pheromone Potential queen Dufour’s gland Reproductive monopoly 



We thank the Department of Science and Technology, the Department of Biotechnology, the Council for Scientific and Industrial Research and the Ministry of Environment and Forests, Government of India for financial assistance, and Robin Crewe for helpful comments and encouragement. We also thank Anjali Rajasekharan, Pooja Muralidharan and Gautam Pramanik, Centre for Ecological Sciences, Indian Institute of Science, Bangalore, for technical assistance with the GC-MS analyses and, Prasanta Das, Department of Inorganic and Physical Chemistry, Indian Institute of Science, Bangalore, for providing useful suggestions regarding interpretation of mass spectra. AB carried out the behavioral observations. SAD, KC, and AM did the dissections and prepared the extracts. AM carried out the chemical analysis, with help and guidance from DGN and AH. The paper was co-written by AB, AM, and RG, and RG supervised the overall work. All experiments reported here comply with the current laws of the country in which they were performed.

Supplementary material

10886_2010_9770_MOESM1_ESM.pdf (34 kb)
Supplementary material S1 Flame ionization detection gas chromatogram of a Ropalidia marginata Dufour’s gland crushed in Ringer’s solution, evaporated to dryness and resuspended in pentane. Asterisk signifies contaminant from Ringer’s solution. Identities of peak numbers are given in Table 1. Compare the chromatogram of this extraction method with that obtained in S2 (PDF 34 kb)
10886_2010_9770_MOESM2_ESM.pdf (189 kb)
Supplementary material S2 Comparison of gas chromatograms of the same sample of a Ropalidia marginata Dufour’s gland extracted in pentane and analyzed by flame ionization detection (FID) and mass spectrometry (total ion chromatogram; TIC). Identities of peak numbers are given in Table 1 (PDF 188 kb)
10886_2010_9770_MOESM3_ESM.pdf (19 kb)
Supplementary material S3 Flame ionization detection gas chromatogram of a Ropalidia marginata Dufour’s gland crushed and extracted in acetone. Identities of peak numbers are given in Table 1. Compare the chromatogram of this extraction method with that obtained in S2 (PDF 19 kb)


  1. Bhadra, A., Iyer, P. L., Sumana, A., Deshpande, S. A., Ghosh, S., and Gadagkar, R. 2007. How do workers of the primitively eusocial wasp Ropalidia marginata detect the presence of their queens? J. Theor. Biol. 246: 574–582.PubMedCrossRefGoogle Scholar
  2. Dapporto, L., Santini, A., Dani, F. R., and Turillazzi, S. 2007. Workers of a Polistes paper wasp detect the presence of their queen by chemical cues. Chem. Senses 32: 795–802. doi: 10.1093/chemse/bjm047.PubMedCrossRefGoogle Scholar
  3. Deshpande, S. A., Sumana, A., Surbeck, M., and Gadagkar, R. 2006. Wasp who would be queen: A comparative study of two primitively eusocial species. Curr. Sci. 91: 332–336.Google Scholar
  4. Dor, R., Katzav-Gozansky, T., and Hefetz, A. 2005. Dufour’s gland pheromone as a reliable fertility signal among honeybee (Apis mellifera) workers. Behav. Ecol. 49: 401–409.Google Scholar
  5. Downing, H. A. 1991. A role of the Dufour's gland in the dominance interactions of the paper wasp, Polistes fuscatus (Hymenoptera: Vespidae). J. Insect Behav. 4: 557–565.CrossRefGoogle Scholar
  6. Gadagkar, R. 2001. The Social Biology of Ropalidia marginata: Toward Understanding the Evolution of Eusociality. Harvard University Press, Cambridge.Google Scholar
  7. Kardile, S. P., and Gadagkar, R. 2002. Docile sitters and active fighters in paper wasps: a tale of two queens. Naturwissenschaften 89: 302–304. doi: 10.1007/s00114-002-0306-2.CrossRefGoogle Scholar
  8. Katzav-Gozansky, T., Soroker, V., and Hefetz, A. 1997. Plasticity of caste specific Dufour’s gland secretion in the honey bee (Apis mellifera L.). Naturwissenschaften 84: 238–241.CrossRefGoogle Scholar
  9. Katzav-Gozansky, T., Soroker, V., and Hefetz, A. 2002. Evolution of worker sterility in honey bees: egg-laying workers express queen-like secretion in Dufour’s gland. Behav. Ecol. Sociobiol. 51: 588–589.CrossRefGoogle Scholar
  10. Keller, L., and Nonacs, P. 1993. The role of queen pheromones in social insects: queen control or queen signal? Anim. Behav. 45: 787–794.CrossRefGoogle Scholar
  11. Krebs, C. J. 1989. Ecological Methodology. Harper & Row, New York.Google Scholar
  12. Premnath, S., Sinha, A., and Gadagkar, R. 1996. Dominance relationships in the establishment of reproductive division of labour in a primitively eusocial wasp (Ropalidia marginata). Behav. Ecol. Sociobiol. 39: 125–132, doi: 10.1007/s002650050274.CrossRefGoogle Scholar
  13. Reyment, R. A. 1989. Compositional data analysis. Terra Nova. 1: 29–34.CrossRefGoogle Scholar
  14. Shannon, C. E. 1948. A mathematical theory of communication. The Bell Syst. Tech. J. 27: 379–423 & 623–656.Google Scholar
  15. Sledge, M. F., Boscaro, F., and Turillazzi, S. 2001. Cuticular hydrocarbons and reproductive status in the social wasp Polistes dominulus. Behav. Ecol. Sociobiol. 58: 270–276.Google Scholar
  16. Sumana, A., and Gadagkar, R. 2003. Ropalidia marginata—a primitively eusocial wasp society headed by behaviourally non-dominant queens. Curr. Sci. 84: 1464–1468.Google Scholar
  17. Sumana, A., Deshpande, S. A., Bhadra, A., and Gadagkar, R. 2007/2008. Workers of the primitively eusocial wasp Ropalidia marginata do not perceive their queen across a wire mesh partition. J. Ethol. 26: 207–212. doi: 10.1007/s10164-007-0049-9.CrossRefGoogle Scholar
  18. Wilson, E. O. 1971. The Insect Societies. The Belknap Press of Harvard University Press, Cambridge.Google Scholar
  19. Zar, J. H. 1999. Biostatistical Analysis. Pearson Education Inc., Tan Prints Private Ltd., India.Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Anindita Bhadra
    • 1
  • Aniruddha Mitra
    • 1
  • Sujata A. Deshpande
    • 1
  • Kannepalli Chandrasekhar
    • 1
  • Dattatraya G. Naik
    • 3
  • Abraham Hefetz
    • 4
  • Raghavendra Gadagkar
    • 1
    • 2
  1. 1.Centre for Ecological SciencesIndian Institute of ScienceBangaloreIndia
  2. 2.Evolutionary and Organismal Biology UnitJawaharlal Nehru Centre for Advanced Scientific ResearchJakkurIndia
  3. 3.Agharkar Research InstitutePuneIndia
  4. 4.Department of ZoologyTel Aviv UniversityTel AvivIsrael

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