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Journal of Chemical Ecology

, Volume 33, Issue 7, pp 1449–1455 | Cite as

Effects of Root Isoquinoline Alkaloids from Hydrastis canadensis on Fusarium oxysporum Isolated from Hydrastis Root Tissue

  • Michael C. Tims
  • Charisma Batista
Article

Abstract

Goldenseal (Hydrastis canadensis L.) is a popular medicinal plant distributed widely in North America. The rhizome, rootlets, and root hairs produce medicinally active alkaloids. Berberine, one of the Hydrastis alkaloids, has shown antifungal activity. The influence of a combination of the major Hydrastis alkaloids on the plant rhizosphere fungal ecology has not been investigated. A bioassay was developed to study the effect of goldenseal isoquinoline alkaloids on three Fusarium isolates, including the two species isolated from Hydrastis rhizosphere. The findings suggest that the Hydrastis root extract influences macroconidia germination, but that only the combined alkaloids—berberine, canadine, and hydrastine—appear to synergistically stimulate production of the mycotoxin zearalenone in the Fusarium oxysporum isolate. The Hydrastis root rhizosphere effect provided a selective advantage to the Fusarium isolates closely associated with the root tissue in comparison with the Fusarium isolate that had never been exposed to Hydrastis.

Keywords

Hydrastis Goldenseal Fusarium F. oxysporum F. solani F. commune Endophyte Isoquinoline alkaloid Rhizosphere Chemical ecology 

Notes

Acknowledgements

I acknowledge Paul Strauss, for permission to wildcraft goldenseal on United Plant Savers land and Alex Johnson for contributions to the ELISA detection. This study is part of the Ph.D. dissertation research undertaken by Michael C. Tims.

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  1. 1.Department of Cell Biology and Molecular GeneticsUniversity of MarylandCollege ParkUSA
  2. 2.F. Hebert School of MedicineUniform Services University of Health SciencesBethesdaUSA
  3. 3.Division of Analytical ChemistryNational Institute of Standards and TechnologyGaithersburgUSA

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