The Influence of Tissue Handling on the Flavonoid Content of the Aquatic Plant Posidonia oceanica
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In recent times, more and more studies have focused on flavonoids as biomarkers of environmental quality in aquatic plants, in particular, Posidonia oceanica (Linnaeus) Delile. It is therefore of interest to determine how different prehandling methods can affect flavonoid concentrations. The methods tested were (1) immediate extraction of fresh samples, (2) extraction after 48 hr chilling, (3) freeze-drying, and (4) oven drying. Chilling and freeze-drying considerably altered the quantity of flavonoids measured, but not their profile. The effect of oven drying was not significant. Chilling led to a loss of 57% of total (pro)anthocyanidins, 39% of total flavonols, and 48% of all simple flavonols (myricetin, quercetin, isorhamnetin, and kaempferol). Freeze-drying caused a loss of 71% of total (pro)anthocyanidins, 87% of total flavonols, and 95% of all simple flavonols.
KeywordsPrehandling methods Chilling Freeze-drying Oven-drying Posidonia oceanica Flavonoids (pro)Anthocyanidins Flavonols
This work was carried out and funded in the framework of the Interreg III European Programs. The authors thank C. Lafabrie and B. Mimault for participation in field missions.
- Chalker-Scott, L., and Fuchigami, L. H. 1989. The role of phenolic compounds in plant stress responses, pp. 67–69, in P. H. Li (ed.). Low Temperature Stress Physiology in Crops. CRC Press, Boca Raton.Google Scholar
- Chalker-Scott, L., Fuchigami, L. H., and Harber, R. M. 1989. Spectrophotometric measurement of leached phenolic compounds as an indicator of freeze damage. J. Am. Soc. Hortic. Sci. 114:315–319.Google Scholar
- Waterman, P. G., and Mole, S. 1994. Analysis of phenolic plant metabolites, p. 231, in J. H Lawron and G. E. Likens (eds.). Methods in Ecology. Blackwell, Oxford.Google Scholar