Beyond 9-ODA: SEX Pheromone Communication in the European Honey Bee Apis mellifera L.
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The major component of the mandibular gland secretion of queen honeybees (Apis mellifera L.), 9-ODA ((2E)-9-oxodecenoic acid), has been known for more than 40 yr to function as a long-range sex pheromone, attracting drones at congregation areas and drone flyways. Tests of other mandibular gland components failed to demonstrate attraction. It remained unclear whether these components served any function in mating behavior. We performed dual-choice experiments, using a rotating drone carousel, to test the attractiveness of 9-ODA compared to mixtures of 9-ODA with three other most abundant components in virgin queen mandibular gland secretions: (2E)-9-hydroxydecenoic acid (9-HDA), (2E)-10-hydroxydecenoic acid (10-HDA), and p-hydroxybenzoate (HOB). We found no differences in the number of drones attracted to 9-ODA or the respective mixtures over a distance. However, adding 9-HDA and 10-HDA, or 9-HDA, 10-HDA, and HOB to 9-ODA increased the number of drones making contact with the baited dummy. On the basis of these results, we suggest that at least 9-HDA and 10-HDA are additional components of the sex pheromone blend of A. mellifera.
Key WordsSex pheromone mating behavior mandibular gland honeybee Apis mellifera (2E)-9-oxodecenoic acid (2E)-9-hydroxydecenoic acid (2E)-10-hydrodecenoic acid
We thank G. Eisenmann and N. Schneider, who built the drone carousel, and K. Galmbacher and P. Bernbauer, who helped with data collection. J.S. and A.B. were supported by the German Research Foundation DFG (SFB554 and Graduiertenkolleg 200).
- Brockmann, A. and Brückner, D. 2005. Drone antennae and the evolution of sex-pheromone communication in honeybees. Indian Bee J. 65:131–138.Google Scholar
- Butler, C. G. 1971. The mating behavior of the honeybee. J. Entomol. (A) 46:1–11.Google Scholar
- Christensen, T. A. 1997. Anatomical and physiological diversity in the central processing of sex pheromone information in different moth species, pp. 184–193. in R.T. Cardé and A. K. Minks (eds.). Insect Pheromone Research. New Directions. Chapman and Hall, New York.Google Scholar
- Free, J. B., 1987. Pheromones of Social Bees. Comstock, Ithaca, NY.Google Scholar
- Loper, G. M., Taylor, O. R. Jr., Foster, L. J., and Kochansky, J. 1996. Relative attractiveness of queen mandibular pheromone components to honey bee (Apis mellifera L.) drones. J. Apic. Res. 35:122–123.Google Scholar
- Phelan, P. L. 1992. Evolution of sex pheromones and the role of asymmetric tracking, pp. 265–314, in B. D. Roitberg and M. B. Ismam (eds.). Insect Chemical Ecology. An Evolutionary Approach. Chapman and Hall, New York.Google Scholar
- Ruttner, F., 1985. Reproductive behaviour in honeybees, pp. 225–236, in B. Hölldobler and M. Lindauer (eds.). Fortschritte der Zoologie, Vol. 31. Gustav Fischer Verlag, Stuttgart.Google Scholar
- Velthuis, H. H. W. 1985. The honeybee queen and the social organization of her colony. Fortschr. Zool. 31:343–357.Google Scholar
- Wilson, E. O. 1971. The Insect Societies. Harvard University Press, Cambridge, MA.Google Scholar