Journal of Chemical Ecology

, Volume 32, Issue 3, pp 595–604 | Cite as

Leaf Epicuticular Wax Chemicals of the Japanese Knotweed Fallopia japonica as Oviposition Stimulants for Ostrinia latipennis



Extraction, fractionation, and gas chromatography−mass spectrometry analyses guided by bioassays have shown that n-alkanes and free fatty acids in leaf epicuticular wax of the Japanese knotweed Fallopia (Reynoutria) japonica stimulate oviposition in the Far-Eastern knotweed borer, Ostrinia latipennis (Lepidoptera: Crambidae). n-Alkanes made up 48.1% of the total amount of epicuticular wax, and their carbon chain length was in the C16−C33 range, with n-nonacosane (n-C29) most abundant, followed by n-C27, n-C25, and n-C31. Free fatty acids with C9−C22 accounted for 22.3%, and hexadecanoic acid was predominant. A mixture of authentic n-alkanes and fatty acids of the composition found in the epicuticular wax, a mixture of n-alkanes, and a mixture of fatty acids significantly enhanced oviposition. Thus, it was demonstrated that both n-alkanes and free fatty acids in leaf epicuticular wax of F. japonica are naturally occurring oviposition stimulants for O. latipennis.

Key Words

Fallopia japonica Ostrinia latipennis alkane fatty acid oviposition stimulant 



A postdoctoral research fellowship to G. Li from the Japanese Society for the Promotion of Science (No. 14-02209) is acknowledged. We thank Dr. S. Tatsuki and Dr. S. Hoshizaki of our laboratory for useful discussions during the course of this research.


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© Springer Science + Business Media, Inc. 2006

Authors and Affiliations

  1. 1.Laboratory of Applied Entomology, Graduate School of Agricultural and Life SciencesThe University of TokyoTokyoJapan
  2. 2.Key Laboratory of Monitoring and Management of Plant Diseases and Pests, Ministry of AgricultureNanjing Agricultural UniversityNanjingChina

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