Leaf Epicuticular Wax Chemicals of the Japanese Knotweed Fallopia japonica as Oviposition Stimulants for Ostrinia latipennis
- 238 Downloads
Extraction, fractionation, and gas chromatography−mass spectrometry analyses guided by bioassays have shown that n-alkanes and free fatty acids in leaf epicuticular wax of the Japanese knotweed Fallopia (Reynoutria) japonica stimulate oviposition in the Far-Eastern knotweed borer, Ostrinia latipennis (Lepidoptera: Crambidae). n-Alkanes made up 48.1% of the total amount of epicuticular wax, and their carbon chain length was in the C16−C33 range, with n-nonacosane (n-C29) most abundant, followed by n-C27, n-C25, and n-C31. Free fatty acids with C9−C22 accounted for 22.3%, and hexadecanoic acid was predominant. A mixture of authentic n-alkanes and fatty acids of the composition found in the epicuticular wax, a mixture of n-alkanes, and a mixture of fatty acids significantly enhanced oviposition. Thus, it was demonstrated that both n-alkanes and free fatty acids in leaf epicuticular wax of F. japonica are naturally occurring oviposition stimulants for O. latipennis.
Key WordsFallopia japonica Ostrinia latipennis alkane fatty acid oviposition stimulant
A postdoctoral research fellowship to G. Li from the Japanese Society for the Promotion of Science (No. 14-02209) is acknowledged. We thank Dr. S. Tatsuki and Dr. S. Hoshizaki of our laboratory for useful discussions during the course of this research.
- De Jesus, L. R. A., Calumpang, S. M. F., Medina, J. R., and Ohsawa, K. 2003. Component fatty acids from green mango fruit surface stimulate oviposition of the mango pulp weevil, Sternochetus frigidus. Philipp. Agric. Sci. 86:38–45.Google Scholar
- Kolattukudy, P. E. 1996. Biosynthetic pathways of cutin and waxes, their sensitivity to environmental stresses, pp. 83–108, in G. Kersteins (ed.), Plant Cuticles, An Integrated Functional Approach, BIOS Scientific Publishers Ltd, Oxford.Google Scholar
- Metcalf, R. L. 1985. Plant kairomones and insect pest control. Ill. Nat. Hist. Surv. Bull. 33:175–196.Google Scholar
- Miyazawa, M. and Kameoka, H. 1976. The constituents of the steam volatile oil from Polygonum reynoutria Makino. Yakushigaku Zasshi 96:1076–1080.Google Scholar
- Mutuura, A. and Munroe, E. 1970. Taxonomy and distribution of the European corn borer and allied species: genus Ostrinia (Lepidoptera: Pyralidae). Mem. Entomol. Soc. Can. 71:1–112.Google Scholar
- Städler, E. 1986. Oviposition and feeding stimuli in leaf surface waxes, pp. 105–112, in B. E. Juniper and T. R. E. Southwood (eds.). Insects and the Plant Surface. Edward Arnold, London.Google Scholar