Leaf Epicuticular Wax Chemicals of the Japanese Knotweed Fallopia japonica as Oviposition Stimulants for Ostrinia latipennis
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Extraction, fractionation, and gas chromatography−mass spectrometry analyses guided by bioassays have shown that n-alkanes and free fatty acids in leaf epicuticular wax of the Japanese knotweed Fallopia (Reynoutria) japonica stimulate oviposition in the Far-Eastern knotweed borer, Ostrinia latipennis (Lepidoptera: Crambidae). n-Alkanes made up 48.1% of the total amount of epicuticular wax, and their carbon chain length was in the C16−C33 range, with n-nonacosane (n-C29) most abundant, followed by n-C27, n-C25, and n-C31. Free fatty acids with C9−C22 accounted for 22.3%, and hexadecanoic acid was predominant. A mixture of authentic n-alkanes and fatty acids of the composition found in the epicuticular wax, a mixture of n-alkanes, and a mixture of fatty acids significantly enhanced oviposition. Thus, it was demonstrated that both n-alkanes and free fatty acids in leaf epicuticular wax of F. japonica are naturally occurring oviposition stimulants for O. latipennis.
Key WordsFallopia japonica Ostrinia latipennis alkane fatty acid oviposition stimulant
A postdoctoral research fellowship to G. Li from the Japanese Society for the Promotion of Science (No. 14-02209) is acknowledged. We thank Dr. S. Tatsuki and Dr. S. Hoshizaki of our laboratory for useful discussions during the course of this research.
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