Abstract
Several Streptomyces species are known to produce metabolites that inhibit plant pathogens. One such compound is geldanamycin (GA), a benzoquinone ansamycin originally isolated from Streptomyces hygroscopicus. We examined the effect of geldanamycin on egg hatch and juvenile motility in Caenorhabditis elegans and in two populations of the plant-parasitic nematode Heterodera glycines. When C. elegans eggs were exposed to geldanamycin, both hatch and motility were reduced by GA doses between 2 and 50 μg/ml. The H. glycines inbred populations TN17 and TN18 exhibited low dose stimulation of hatch and motility, whereas levels occurring at higher GA doses were at or below control levels. These experiments represent the first demonstration of geldanamycin effects in C. elegans and H. glycines and suggest that the heat shock chaperone Hsp90, the known molecular target of geldanamycin, may be involved in nematode egg hatch and motility. This study also indicates that geldanamycin-producing strains of Streptomyces may be useful as biocontrol agents for nematodes.
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Acknowledgments
The authors thank Maria Hult, Paula Crowley, Sharon Ochs, and Steven Rogers for technical assistance, and Dr. Terry Niblack for strains TN17 and TN18. This study was supported in part by grant #1241 from the United Soybean Board. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture.
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Skantar, A.M., Agama, K., Meyer, S.L.F. et al. Effects of Geldanamycin on Hatching and Juvenile Motility in Caenorhabditis elegans and Heterodera glycines. J Chem Ecol 31, 2481–2491 (2005). https://doi.org/10.1007/s10886-005-7114-z
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DOI: https://doi.org/10.1007/s10886-005-7114-z