Journal of Chemical Ecology

, Volume 31, Issue 9, pp 2019–2032 | Cite as

The Involvement of Volatile Infochemicals from Spider Mites and from Food-Plants in Prey Location of the Generalist Predatory Mite Neoseiulus californicus

  • Takeshi Shimoda
  • Rika Ozawa
  • Kota Sano
  • Eizi Yano
  • Junji Takabayashi


We investigated volatile infochemicals possibly involved in location of the generalist predatory mite Neoseiulus californicus to plants infested with spider mites in a Y-tube olfactometer. The predators significantly preferred volatiles from lima bean leaves infested with Tetranychus urticae to uninfested lima bean leaves. Likewise, they were attracted to volatiles from artificially damaged lima bean leaves and those from T. urticae plus their visible products. Significantly more predators chose infested lima bean leaves from which T. urticae plus their visible products had been removed than artificially damaged leaves, T. urticae, and their visible products. These results suggest that N. californicus is capable of exploiting a variety of volatile infochemicals originating from their prey, from the prey-foodplants themselves, and from the complex of the prey and the host plants (e.g., herbivore-induced volatiles). We also investigated predator response to some of the synthetic samples identified as volatile components emitted from T. urticae-infested lima bean leaves and/or artificially damaged lima bean leaves. The predators were attracted to each of the five synthetic volatile components: linalool, methyl salicylate, (Z)-3-hexen-1-ol, (E)-2-hexenal, and (Z)-3-hexenyl acetate. The role of each volatile compound in prey-searching behavior is discussed.

Key Words

Neoseiulus californicus Tetranychus urticae lima bean herbivore-induced volatiles green leaf volatiles 



We thank Kimiko Kanbe and Yumiko Togashi for rearing the mites and plants. We also thank Dr. Yoshito Suzuki and Dr. Chie Goto for constructive comments during the study. This study was supported by the Bio-oriented Technology Research Advancement Institution.


  1. Arimura, G., Ozawa, R., Shimoda, T., Nishioka, T., Boland, W., Takabayashi, J. 2000Herbivory-induced volatiles elicit defense genes in lima bean leavesNature406512515CrossRefPubMedGoogle Scholar
  2. Croft, B. A., Monetti, L. N., Pratt, P. D. 1998Comparative life histories and predation types: Are Neoseiulus californicus and N. fallacis (Acari: Phytoseiidae) similar type II selective predators of spider mites?Environ. Entomol.27531538Google Scholar
  3. Dicke, M. 1999Are herbivore-induced plant volatiles reliable indicators of herbivore identity to foraging carnivorous arthropods?Entomol. Exp. Appl.91131142Google Scholar
  4. Dicke, M. 1999Evolution of induced indirect defense of plantsTollrian, R.Harvell, C. D. eds. The Ecology and Evolution of Inducible DefensePrinceton University PressPrinceton6388Google Scholar
  5. Dicke, M., Beek, T. A., Posthumus, M. A., Ben Don, N., Bokhoven, H., Groot, A. E. 1990Isolation and identification of volatile kairomone that affects acarine predator–prey interactions: Involvement of host plant in its productionJ. Chem. Ecol.16381396CrossRefGoogle Scholar
  6. Dicke, M., Takabayashi, J., Posthumus, M. A., Schütte, C., Olga, E. K. 1998Plant–phytoseiid interactions mediated by herbivore-induced plant volatiles: Variation in production of cues and in responses of predatory mitesExp. Appl. Acarol.22311333CrossRefGoogle Scholar
  7. Drukker, B., Bruin, J., Sabelis, M. W. 2000Anthocorid predators learn to associate herbivore-induced plant volatiles with presence or absence of preyPhysiol. Entomol.25260265CrossRefGoogle Scholar
  8. Dwumfour, E. F. 1992Volatile substances evoking orientation in the predatory flowerbug Anthocoris nemorum (Heteroptera: Anthocoridae)Bull. Entomol. Res.82465469Google Scholar
  9. Easterbrook, M. A., Fitzgerald, J. D., Solomon, M. G. 2001Biological control of strawberry tarsonemid mite Phytonemus pallidus and two-spotted spider mite Tetranychus urticae on strawberry in the UK using species of Neoseiulus (Amblyseius) (Acari: Phytoseiidae)Exp. Appl. Acarol.252536CrossRefPubMedGoogle Scholar
  10. Garcia-Mari, F., Gonzalez-Zamora, J. E. 1999Biological control of Tetranychus urticae (Acari: Tetranychidae) with naturally occurring predators in strawberry plantings in Valencia, SpainExp. Appl. Acarol.23487495CrossRefGoogle Scholar
  11. Greco, N. M., Liljesthrom, C. G., Sanchez, N. E. 1999Spatial distribution and coincidence of Neoseiulus californicus and Tetranychus urticae (Acari: Phytoseiidae, Tetranychidae) on strawberryExp. Appl. Acarol.23567580CrossRefGoogle Scholar
  12. James, D. G. 2003Synthetic herbivore-induced plant volatiles as field attractants for beneficial insectsEnviron. Entomol.32977982Google Scholar
  13. James, D. G., Price, T. S. 2004Field-testing of methyl salicylate for recruitment and retention of beneficial insects in grapes and hopsJ. Chem. Ecol.3016131628CrossRefPubMedGoogle Scholar
  14. Janssen, A., Hofker, C. D., Braun, A. R., Mesa, N., Sabelis, M. W., Bellotti, A. C. 1990Preselecting predatory mites for biological control: the use of an olfactometerBull. Entomol. Res.80177181Google Scholar
  15. Lewis, W. J., Martin, W. R.,Jr. 1990Semiochemicals for use with parasitoids: Status and futureJ. Chem. Ecol.1630673089CrossRefGoogle Scholar
  16. Llusia, J., Penuelas, J. 2001Emission of volatile organic compounds by apple trees under spider mite attack and attraction of predatory mitesExp. Appl. Acarol.256577CrossRefPubMedGoogle Scholar
  17. Maeda, T., Takabayashi, J., Yano, S., Takafuji, A. 1999Response of the predatory mite, Amblyseius womersleyi (Acari: Phytoseiidae), toward herbivore-induced plant volatiles: Variation in response between two local populationsAppl. Entomol. Zool.34449454Google Scholar
  18. Ozawa, R., Arimura, G., Takabayashi, J., Shimoda, T., Nishioka, T. 2000Involvement of jasmonic- and salicylate-related signaling pathways for the production of specific herbivore-induced volatiles in plantPlant Cell Physiol.41391398PubMedGoogle Scholar
  19. Reddy, G. V. P., Holopainen, J. K., Guerrero, A. 2002Olfactory responses of Plutella xylostella natural enemies to host pheromone, larval frass, and green leaf cabbage volatilesJ. Chem. Ecol.28131143CrossRefPubMedGoogle Scholar
  20. Sabelis, M. W., Baan, H. E. 1983Location of distant spider mite colonies by phytoseiid predators: Demonstration of specific kairomones emitted by Tetranychus urticae and Panonychus ulmiEnt. Exp. Appl.33303314Google Scholar
  21. Sabelis, M. W., Afman, B. P., Slim, P. J. 1984Location of distant spider mite colonies by Phytoseiulus persimilis: Localization and extraction of a kairomoneGriffiths, D. A.Bowman, C. E. eds. Acarology VI, Vol. 1Ellis HorwoodChichester, UK431440Google Scholar
  22. Scutareanu, P., Drukker, B., Bruin, J., Posthumus, M. A., Sabelis, M. W. 1997Volatiles from Psylla-infested pear trees and their possible involvement in attraction of anthocorid predatorsJ. Chem. Ecol.2322412260CrossRefGoogle Scholar
  23. Shimoda, T., Takabayashi, J. 2001Response of Oligota kashmirica benefica, a specialist insect predator of spider mites, to volatiles from prey-infested leaves under both laboratory and field conditionsEntomol. Exp. Appl.14147CrossRefGoogle Scholar
  24. Shimoda, T., Takabayashi, J., Ashihara, W., Takafuji, A. 1997Response of predatory insect Scolothrips takahashii toward herbivore-induced plant volatiles under laboratory and field conditionsJ. Chem. Ecol.2320332048CrossRefGoogle Scholar
  25. Shiojiri, K., Maeda, T., Arimura, G., Ozawa, R., Shimoda, T., Takabayashi, J. 2002Functions of plant infochemicals in tritrophic interactions between plants, herbivores and carnivorous natural enemiesJpn. J. Appl. Entomol. Zool.46117133CrossRefGoogle Scholar
  26. Sokal, R. R., Rohlf, F. J. 1998BiometryFreemanNew YorkGoogle Scholar
  27. Steinberg, S., Dicke, M., Vet, L. E. M. 1993Relative importance of infochemicals from first and second trophic level in long-range host location by the larval parasitoid Cotesia glomerataJ. Chem. Ecol.194759CrossRefGoogle Scholar
  28. Takabayashi, J., Dicke, M. 1992Response of predatory mites with different rearing histories to volatiles of uninfested plantsEntomol. Exp. Appl.64187193Google Scholar
  29. Takabayashi, J., Dicke, M. 1996Plant–carnivore mutualism through herbivore-induced carnivore attractantsTrends Plant Sci.1109113CrossRefGoogle Scholar
  30. Whitman, D. W., Eller, F. J. 1992Orientation of Microplitis croceipes (Hymenoptera: Braconidae) to green leaf volatiles: Dose–response curvesJ. Chem. Ecol.1817431753Google Scholar

Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Takeshi Shimoda
    • 1
  • Rika Ozawa
    • 2
    • 3
  • Kota Sano
    • 4
  • Eizi Yano
    • 1
  • Junji Takabayashi
    • 2
    • 3
  1. 1.Insect Biocontrol Laboratory, Department of Entomology and NematologyNational Agricultural Research CenterTsukubaJapan
  2. 2.Center of Ecological ResearchKyoto UniversityOtsuJapan
  3. 3.CREST of Japan Science and Technology CooperationTokyoJapan
  4. 4.Fragrance LaboratorySoda Aromatic Co. Ltd.TokyoJapan

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