Nanoparticles of Punicalagin Synthesized from Pomegranate (Punica Granatum L.) with Enhanced Efficacy Against Human Hepatic Carcinoma Cells

Abstract

Punicalagin is the most popular ellagitannin found in pomegranate husk and is well known to reduce the risk of cancer and cardiovascular diseases (CVDs). The present work describes a novel method for the preparation of nanoparticles of Punicalagin using pomegranate (Punica granatum) since the use of Punicalagin in bulk form is associated with many problems. In this study, nanoparticles of Punicalagin were synthesized, characterized using various techniques, and further evaluated for their antioxidant, antibacterial, and antiproliferative potential using HepG2 cancer cells. DLS analysis revealed that the average size of nanoparticles of Punicalagin was 87 nm, whereas SEM analysis confirmed spherical shaped nanoparticles with size ranging from 90 to 116 nm. HPLC studies confirmed the presence of Punicalagin in synthesized nanoparticles. The results also revealed that nanoparticles of Punicalagin were nearly four times more potent antioxidants than bulk and possessed an inhibition zone of about 13 mm. The response of antiproliferative assay showed that the nanoparticles of Punicalagin caused nearly 44% reduction while bulk form showed only a 15% reduction in cell viability of cancerous cells at 100 µg/mL. The study suggests a great potential for use of the herbal drug in nano form to treat cancer as compared to its bulk counterparts.

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References

  1. 1.

    M. Russo, C. Fanali, G. Tripodo, P. Dugo, R. Muleo, L. Dugo, L. De Gara, and L. Mondello (2018). Anal. Bioanal. Chem. https://doi.org/10.1007/s00216-018-0854-8.

    Article  PubMed  Google Scholar 

  2. 2.

    S. Sreekumar, H. Sithul, P. Muraleedharan, J. M. Azeez, and S. Sreeharshan (2014). BioMed. Res. Int. https://doi.org/10.1155/2014/686921.

    Article  PubMed  PubMed Central  Google Scholar 

  3. 3.

    M. Aviram and M. Rosenblat (2013). Rambam Maimonides Med. J. https://doi.org/10.5041/RMMJ.10113.

    Article  PubMed  PubMed Central  Google Scholar 

  4. 4.

    X. Lu: Incorporation of mushroom powder into cereal food products. Lincoln University (2018)

  5. 5.

    N. M. Badawi, M. H. Teaima, K. M. El-Say, D. A. Attia, M. A. El-Nabarawi, and M. M. Elmazar (2018). Int. J. Nanomed. https://doi.org/10.2147/IJN.S154033.

    Article  PubMed  PubMed Central  Google Scholar 

  6. 6.

    V. Sanna, I. A. Siddiqui, M. Sechi, and H. Mukhtar (2013). Cancer Lett. https://doi.org/10.1016/j.canlet.2012.11.037.

    Article  PubMed  Google Scholar 

  7. 7.

    A. B. Shirode, D. J. Bharali, S. Nallanthighal, J. K. Coon, S. A. Mousa, and R. Reliene (2015). Int. J. Nanomed. https://doi.org/10.2147/IJN.S65145.

    Article  PubMed  PubMed Central  Google Scholar 

  8. 8.

    I. A. Siddiqui, V. M. Adhami, D. J. Bharali, B. B. Hafeez, M. Asim, S. I. Khwaja, N. Ahmad, H. Cui, S. A. Mousa, and H. Mukhtar (2009). Cancer Res. https://doi.org/10.1158/0008-5472.CAN-08-3978.

    Article  PubMed  PubMed Central  Google Scholar 

  9. 9.

    M. Gera, R. Kumar, V. Jain, Adv. Sci. Eng. Med. (2015) https://doi.org/10.1166/asem.2015.1722

  10. 10.

    A. Mehra, R. Narang, V. Jain, and S. Nagpal (2020). Eur. J. Integr. Med. https://doi.org/10.1016/j.eujim.2019.101014.

    Article  Google Scholar 

  11. 11.

    E.A. Alamineh, Am. J. Appl. Chem. (2018) https://doi.org/10.11648/j.ajac.20180602.13

  12. 12.

    J. Lu, K. Ding, and Q. Yuan (2008). Chromatographia. https://doi.org/10.1365/s10337-008-0699-y.

    Article  PubMed  PubMed Central  Google Scholar 

  13. 13.

    P. M. Carvalho, M. R. Felício, N. C. Santos, S. Gonçalves, and M. M. Domingues (2018). Front. Chem. https://doi.org/10.3389/fchem.2018.00237.

    Article  PubMed  PubMed Central  Google Scholar 

  14. 14.

    D. N. de Assis, V. C. F. Mosqueira, J. M. C. Vilela, M. S. Andrade, and V. N. Cardoso (2008). Int. J. Pharm. https://doi.org/10.1016/j.ijpharm.2007.08.002.

    Article  PubMed  Google Scholar 

  15. 15.

    K. Jores, W. Mehnert, M. Drechsler, H. Bunjes, C. Johann, and K. Mäder (2004). J. Controlled Release. https://doi.org/10.1016/j.jconrel.2003.11.012.

    Article  PubMed  Google Scholar 

  16. 16.

    G. Socrates: Infrared and Raman characteristic group frequencies: tables and charts. John Wiley & Sons, (2004)

  17. 17.

    R. Bhargava and I. W. Levin (2001). Anal. Chem. https://doi.org/10.1021/ac010380m.

    Article  PubMed  Google Scholar 

  18. 18.

    M. Piecha, M. Sarakha, P. Trebše, and D. Kočar (2010). Environ. Chem. Lett. https://doi.org/10.1007/s10311-009-0207-0.

    Article  Google Scholar 

  19. 19.

    M. Balouiri, M. Sadiki, and S. K. Ibnsouda (2016). J. Pharm. Anal. https://doi.org/10.1016/j.jpha.2015.11.005.

    Article  PubMed  Google Scholar 

  20. 20.

    S.S. Dahham, M.N. Ali, H. Tabassum, M. Khan, Am. Eurasian J. Agric. Environ. Sci. (2010)

  21. 21.

    J. Jacob, P. Lakshmanapermalsamy, R. Illuri, D. Bhosle, G.K. Sangli, D. Mundkinajeddu, Pharmacognosy Res. (2018)

  22. 22.

    D.D. Sylvie, P.C. Anatole, B.P. Cabral, P.B. Veronique, Asian Pac. J. Trop. Biomed. (2014) https://doi.org/10.12980/APJTB.4.201414B168

  23. 23.

    L. J. Lalitha, T. J. Sales, P. P. Clarance, P. Agastian, Y. Kim, A. Mahmoud, S. E. Mohamed, J. Tack, S. Na, and H. Kim (2020). J. King Saud Univ.-Sci. https://doi.org/10.1016/j.jksus.2019.11.022.

    Article  Google Scholar 

  24. 24.

    M.F. Abu Bakar, N.E. Ahmad, M. Suleiman, A. Rahmat, A. Isha, Biomed Res Int. (2015) https://doi.org/10.1155/2015/916902

  25. 25.

    W. Sajjad, M. Sohail, B. Ali, A. Haq, G. Din, M. Hayat, I. Khan, M. Ahmad, S. Khan, Mycopath. (2015) https://doi.org/10.21786/bbrc/12.4/38

  26. 26.

    A. Aloqbi, U. Omar, M. Yousr, M. Grace, M. A. Lila, and N. Howell (2016). Nat. Sci. https://doi.org/10.4236/ns.2016.86028.

    Article  Google Scholar 

  27. 27.

    I. Khalil, W.A. Yehye, A.E. Etxeberria, A.A. Alhadi, S.M. Dezfooli, N.B.M. Julkapli, W.J. Basirun, A. Seyfoddin, Antioxidants. (2020)

  28. 28.

    N. Panth, B. Manandhar, K.R. Paudel, Phytother. Res. (2017)

  29. 29.

    B. Aribi, S. Zerizer, Z. Kabouche, I. Screpanti, and R. Palermo (2016). Food Agric. Immunol. https://doi.org/10.1080/09540105.2015.1104654.

    Article  Google Scholar 

  30. 30.

    T. Ghazanfari, M. Naseri, J. Shams, B. Rahmati, Food Agric. Immunol. (2013)

  31. 31.

    V.R. Lombardi, I. Carrera, R. Cacabelos, Evid.-Based Complement. Altern. Med. (2017)

  32. 32.

    J. Li, G. Wang, C. Hou, J. Li, Y. Luo, B. Li, Food Agric. Immunol. (2019)

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Acknowledgements

This research is supported by Amity University, Noida, Uttar Pradesh, and is used by Ms. Akansha Mehra as a part of her Ph.D. program. We would also like to show our gratitude to Dr. Ashok K Chauhan, Founder President, Amity University for his continuous encouragement and support. We are thankful to our host institution also for providing us with resources and instrumentation to carry out this study.

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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AM: Writing-original draft, Writing-review &editing, Methodology, Data Curation. SC: Methodology, Data Analysis, Data Validation, Provision of resources, Visualization. VKJ: Data Validation, Provision of resources, Conceptualization. SN: Supervision, Conceptualization, Investigation of Methodology, Writing-editing, Data Validation, Visualization.

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Correspondence to Suman Nagpal.

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Mehra, A., Chauhan, S., Jain, V.K. et al. Nanoparticles of Punicalagin Synthesized from Pomegranate (Punica Granatum L.) with Enhanced Efficacy Against Human Hepatic Carcinoma Cells. J Clust Sci (2021). https://doi.org/10.1007/s10876-021-01979-9

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Keywords

  • Punicalagin nanoparticles (PCN)
  • Punica granatum
  • Antibacterial
  • Antioxidant
  • HepG2 cancer cells
  • In-vitro antiproliferative activity