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In Vitro Biocidal Actions of Rhus verniciflua Bark Extract Wrapped Gold Nanoballs Against Biofilm-Forming Food-Borne Bacterial Pathogens

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Abstract

The research for innovative antibiofilm drugs is essential due to the increased microbial resistance to antibiotics presently in use. Now a day’s natural products wrapped nano biomaterial play an important role in the field of medicine. The aqueous bark extracts of Rhus verniciflua was used to synthesize gold nanoparticles (Rh-AuNPs) by green method. The synthesized nanoparticles were characterized (optical property, crystallinity, functional groups, size, shape and purity) by UV–vis spectroscopy (UV), X-ray diffraction (XRD), Fourier transform infrared (FTIR), Field emission transmission electron microscopy (FE-TEM) with Energy dispersive X-ray (EDX). An effective antioxidant activity was observed at 100 μg/ml of Rh-AuNPs. The acridine orange, live and dead (PI-SYTO-9) staining assay using confocal laser scanning microscopic,   results evidanced that the Rh-AuNPs (100 μg/ml) was effectively inhibited the biofilms of Escherichia coli and Staphylococcus aureus. In addition, Rh-AuNPs also significantly inhibited the cell surface hydrophobicity of both E. coli and S. aureus at 100 μg/ml. This study reports antibacterial potency of Rh-AuNPs and that have significant therapeutic properties.

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Acknowledgements

This work was supported by Ministry of Agriculture Food and rural Affairs (318077-2). The second author KS thanks the Korea Research Fellowship (KRF) Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT (2017H1D3A1A01052610).

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  1. Natural Product Biomedical Technology Laboratory, Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon, Gangwon-do, 24341, Republic of Korea

    Sekar Vijayakumar, Kandasamy Saravanakumar, Xiaowen Hu & Myeong-Hyeon Wang

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  1. Sekar Vijayakumar
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  2. Kandasamy Saravanakumar
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  3. Xiaowen Hu
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  4. Myeong-Hyeon Wang
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Correspondence to Myeong-Hyeon Wang.

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Vijayakumar, S., Saravanakumar, K., Hu, X. et al. In Vitro Biocidal Actions of Rhus verniciflua Bark Extract Wrapped Gold Nanoballs Against Biofilm-Forming Food-Borne Bacterial Pathogens. J Clust Sci 30, 1489–1499 (2019). https://doi.org/10.1007/s10876-019-01592-x

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