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Plant-Mediated Synthesis, Characterization and Bactericidal Potential of Emerging Silver Nanoparticles Using Stem Extract of Phyllanthus pinnatus: A Recent Advance in Phytonanotechnology

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Abstract

The eco-friendly and cost-effective strategies for the preparation of nanoparticles are the cutting edge researches in the field of nanotechnology. The objective of the study was the eco-friendly synthesis, characterization and their antibacterial activity of AgNPs using stem extract of Phyllanthus pinnatus. The appearance of the dark brown color showed the biofabrication of AgNPs and its characterised by UV–Vis, SEM, XRD, and FT-IR analysis. Consequently, the antibacterial activity of AgNPs was tested against different bacterial pathogens. The UV–Vis spectrum of colloidal AgNPs exhibited a strong absorption peak at 490 nm. Besides, the SEM image showed that the AgNPs were fairly uniform in the nanoscale with mostly cubical morphology. The XRD analysis showed the crystalline nature of AgNPs. In addition, the FT-IR spectrum revealed the presence of functional groups attached to the surface of AgNPs that may have a role in bioreduction and stabilization of AgNPs. The AgNPs showed dose-dependent toxicity against bacterial pathogens. The maximum zone of inhibition was found at 1.8 mm in diameter for both Vibrio cholera and Shigella flexneri at 40 µL concentration. Although the current study showed potent in vitro antibacterial activity of AgNPs, further studies are required to establish the bactericidal potential of AgNPs on animal models for drug development.

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  • 31 March 2020

    In the original publication of this article, co-author name was missed to include in the author group.

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Acknowledgements

The authors thankfully acknowledge the Sathyabama Institute of Science and Technology, Mekelle University, UGC [No.F.14-13/2013, Inno/ASIST], DST-FIST [SR/FST/LSI-667/2016(C)] and MHRD-RUSA 2.0 [F.24/51/2014-U, Policy (TNMulti-Gen), Dept. of Edn. Govt. of India] for the financial supports and infrastructure facilities.

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Authors and Affiliations

  1. Department of Biotechnology, Aarupadiveedu Institute of Technology, Vinayaga Mission’s Research Foundation, Paiyanoor, Chennai, Tamil Nadu, India

    Ramalingam Balachandar & Ramasamy Subbaiya

  2. Department of Biotechnology, Arulmigu Meenakshi Amman College of Engineering, Tiruvanamalai District, Tamil Nadu, India

    Paramasivam Gurumoorthy

  3. Department of Botany, Government Arts College, Salem, Tamil Nadu, 636 007, India

    Natchimuthu Karmegam

  4. Department of Pharmaceutical Biotechnology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Hamed Barabadi

  5. Discipline of Medical Biochemistry and Chemical Pathology, University of KwaZulu-Natal, Durban, South Africa

    Krishnan Anand

  6. Department of Bioinformatics, Alagappa University, Karaikudi, Tamil Nadu, India

    Pandi Boomi

  7. Department of Medical Microbiology and Immunology, Division of Biomedical Science, School of Medicine, College of Health Sciences, Mekelle University, 1871, Mekelle, Ethiopia

    Muthupandian Saravanan

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  1. Ramalingam Balachandar
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Balachandar, R., Gurumoorthy, P., Karmegam, N. et al. Plant-Mediated Synthesis, Characterization and Bactericidal Potential of Emerging Silver Nanoparticles Using Stem Extract of Phyllanthus pinnatus: A Recent Advance in Phytonanotechnology. J Clust Sci 30, 1481–1488 (2019). https://doi.org/10.1007/s10876-019-01591-y

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