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Novel and Facile Synthesis of Sea Anemone Adhesive Protein-Coated ZnO Nanoparticles: Antioxidant, Antibiofilm, and Mosquito Larvicidal Activity Against Aedes aegypti

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Abstract

Recently, marine bio-products have provided a new insight on biomaterials for pharmaceutical applications. Herein, the use of adhesive protein in the fabrication of bio-inspired nanomaterials noted to the possible realization of vital biomedical applications. This study illustrates the biosynthesize ZnO nanoparticles (ZnO NPs) using adhesive protein from sea anemone Stichodactyla haddoni (ShAp). ShAp-ZnO NPs were characterized via SDS-PAGE, UV–Visible, XRD, FTIR, TEM and SAED. The molecular weight of the adhesive protein was determined by SDS-PAGE to be between 36 and 42 kDa. The UV–Vis peak of ShAp-ZnO NPs was revealed at 210 nm while XRD exhibited the crystalline nature of ShAp-ZnO NPs. FTIR revealed that functional group of ShAp-ZnO NPs, which exhibited peaks at 3441.31–1073.36 cm−1. HR-TEM revealed that ShAp-ZnO NPs obtained structure were hexagonal with 10 nm diameter. The antioxidant properties of ShAp, zinc acetate, and ShAp-ZnO NPs were noted at 100 µg/mL. Further, microscopic analysis demonstrated that 50 µg/mL of ShAp, zinc acetate, and ShAp-ZnO NPs efficiently repressed the biofilm formation of both Gram(+) and Gram(−) bacteria. In addition, maximal larvicidal effects were noticed at 100 µg/mL of ShAp-ZnO NPs against the 3rd instar of Aedes aegypti. Overall, the ShAp-ZnO NPs could have entomological and pharmaceutical applications.

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Acknowledgements

The authors appreciatively acknowledge the financial support of RUSA-Phase 2.0-F.24-51/2014-U (TN Multi-Gen), Dept. of Edn, Govt of India. MA gratitude the support of DST-INSPIRE fellowship-IF160623, New Delhi, India. The authors extend their appreciation to the Deanship of Scientific Research at King Saud University for funding this work through research group No. RG-1438-074. The authors thank the Deanship of Scientific Research and RSSU at King Saud University for their technical support.

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Authors and Affiliations

  1. Nano Biosciences and Nano Pharmacology Division, Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health and Management, Alagappa University, Science Block 6th Floor, Burma Colony, Karaikudi, Tamil Nadu, 630004, India

    Muthukumar Abinaya, Ravichandran Rekha & Baskaralingam Vaseeharan

  2. Centre for Animal Science Research and Extension Services, Foundation for Innovative Research in Science and Technology, Kelavannanvilai, NGO Colony Road, Nagercoil, Tamil Nadu, India

    Shanthini Sivakumar

  3. Unit of Vector Control, Phytochemistry and Nanotechnology, Department of Zoology, Annamalai University, Annamalainagar, Tamil Nadu, 608 002, India

    Marimuthu Govindarajan

  4. Department of Zoology, Government College for Women (Autonomous), Kumbakonam, Tamil Nadu, 612 001, India

    Marimuthu Govindarajan

  5. Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia

    Naiyf S. Alharbi, Shine Kadaikunnan, Jamal M. Khaled, Ahmed S. Alobaidi & Mohammed N. Al-Anbr

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  1. Muthukumar Abinaya
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  2. Ravichandran Rekha
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Abinaya, M., Rekha, R., Sivakumar, S. et al. Novel and Facile Synthesis of Sea Anemone Adhesive Protein-Coated ZnO Nanoparticles: Antioxidant, Antibiofilm, and Mosquito Larvicidal Activity Against Aedes aegypti. J Clust Sci 30, 1393–1402 (2019). https://doi.org/10.1007/s10876-019-01581-0

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