Journal of Clinical Immunology

, Volume 32, Issue 6, pp 1372–1380 | Cite as

Colostrum of Healthy Mothers Contains Broad Spectrum of Secretory IgA Autoantibodies

  • Jaroslava Pribylova
  • Klara Krausova
  • Ingrid Kocourkova
  • Pavel Rossmann
  • Klara Klimesova
  • Miloslav Kverka
  • Helena Tlaskalova-Hogenova



Human colostrum and milk provide a newborn with immunomodulatory components, ensuring protection and proper development of the immune system. Secretory IgA antibodies in colostrum represent the first line of defence against harmful substances, but their potential spectra of reactivity with autoantigens remains unclear. Here, we characterised the repertoire of natural sectretory IgA autoantibodies in colostrum of healthy mothers.


The human colostrum samples from 39 healthy mothers were analyzed for autoantibodies by indirect immunofluorescence, dot blots, immunoblots and ELISA.


We found that there is high diversity in reactivities of colostral IgA antibodies to autoantigens among individual samples. Using tissue sections and biochips commonly used for autoimmunity testing, we found that most samples reacted with monkey ovary (79.3 %), monkey pancreatic tissue (78.6 %), human HEp-2 cells (69 %) and monkey adrenal gland (69.0 %), fewer samples reacted with monkey liver tissue (47.2 %), rat stomach (42.9 %), monkey testicular tissue (41.4 %), monkey salivary gland (39.3 %), rat kidney (32.1 %) and monkey cerebellar tissue (17.9 %). At the protein level, we detected reactivity of IgA with 21 out of 25 (auto) antigens. The majority of the samples reacted with the pyruvate dehydrogenase complex, E3 ubiquitin ligase, cytosolic liver antigen, promyelocytic leukemia protein and nuclear pore glycoprotein-210. Using ELISA, we found reactivity of colostral IgA antibodies against examined extractable nuclear antigens, double stranded DNA, phospholipids and neutrophil cytoplasm.


The broad spectrum of polyreactive natural autoantibodies present in human colostrum may contribute to proper development of mucosal immune system of the breastfed infant.


Autoantibodies mucosal immunity immunoglobulins immunohistochemistry/immunohistology anti-DNA antibodies 



secretory IgA


colostral IgA


phosphate-buffered saline


sodium thiocyanate



This work was supported by grants: IAA500200910 (Grant Agency of the Academy of Sciences of the Czech Republic), P304/11/1252 (Czech Science Foundation), and RVO: 61388971 (Institutional Research Concept).

Supplementary material

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ESM 1 (PDF 4356 kb)


  1. 1.
    Ogra PL, Losonsky GA, Fishaut M. Colostrum-derived immunity and maternal-neonatal interaction. Ann N Y Acad Sci. 1983;409:82–95.PubMedCrossRefGoogle Scholar
  2. 2.
    Hanson LA. Session 1: Feeding and infant development breast-feeding and immune function. Proc Nutr Soc. 2007;66(3):384–96. doi: 10.1017/S0029665107005654.PubMedCrossRefGoogle Scholar
  3. 3.
    Tlaskalova-Hogenova H, Vetvicka V, Lodinova R, Cerna J. Immunological components of colostrum and milk; their origin and function. Sci Tech Froid Refrig Sci Technol. 1981;2:133–42.Google Scholar
  4. 4.
    Grumach AS, Carmona RC, Lazarotti D, Ribeiro MA, Rozentraub RB, Racz ML, et al. Immunological factors in milk from Brazilian mothers delivering small-for-date term neonates. Acta Paediatr. 1993;82(3):284–90.PubMedCrossRefGoogle Scholar
  5. 5.
    Wold AE, Mestecky J, Tomana M, Kobata A, Ohbayashi H, Endo T, et al. Secretory immunoglobulin A carries oligosaccharide receptors for Escherichia coli type 1 fimbrial lectin. Infect Immun. 1990;58(9):3073–7.PubMedGoogle Scholar
  6. 6.
    Casas R, Bottcher MF, Duchen K, Bjorksten B. Detection of IgA antibodies to cat, beta-lactoglobulin, and ovalbumin allergens in human milk. J Allergy Clin Immunol. 2000;105(6 Pt 1):1236–40.PubMedCrossRefGoogle Scholar
  7. 7.
    Mestecky J, Russell MW. Specific antibody activity, glycan heterogeneity and polyreactivity contribute to the protective activity of S-IgA at mucosal surfaces. Immunol Lett. 2009;124(2):57–62. doi: 10.1016/j.imlet.2009.03.013.PubMedCrossRefGoogle Scholar
  8. 8.
    Hanson L. Immunobiology of human milk: how breastfeeding protects babies. Amarillo: Pharmasoft Publishing; 2004.Google Scholar
  9. 9.
    Savilahti E, Tainio VM, Salmenpera L, Arjomaa P, Kallio M, Perheentupa J, et al. Low colostral IgA associated with cow’s milk allergy. Acta Paediatr Scand. 1991;80(12):1207–13.PubMedCrossRefGoogle Scholar
  10. 10.
    Tlaskalova-Hogenova H, Tuckova L, Lodinova-Zadnikova R, Stepankova R, Cukrowska B, Funda DP, et al. Mucosal immunity: its role in defense and allergy. Int Arch Allergy Immunol. 2002;128(2):77–89.PubMedCrossRefGoogle Scholar
  11. 11.
    Brandtzaeg P. Induction of secretory immunity and memory at mucosal surfaces. Vaccine. 2007;25(30):5467–84. doi: 10.1016/j.vaccine.2006.12.001.PubMedCrossRefGoogle Scholar
  12. 12.
    Monteiro RC. Role of IgA and IgA Fc receptors in inflammation. J Clin Immunol. 2010;30(1):1–9. doi: 10.1007/s10875-009-9338-0.PubMedCrossRefGoogle Scholar
  13. 13.
    Wines BD, Hogarth PM. IgA receptors in health and disease. Tissue Antigens. 2006;68(2):103–14. doi: 10.1111/j.1399-0039.2006.00613.x.PubMedCrossRefGoogle Scholar
  14. 14.
    Lodinova-Zadnikova R, Slavikova M, Tlaskalova-Hogenova H, Adlerberth I, Hanson LA, Wold A, et al. The antibody response in breast-fed and non-breast-fed infants after artificial colonization of the intestine with Escherichia coli O83. Pediatr Res. 1991;29(4 Pt 1):396–9.PubMedCrossRefGoogle Scholar
  15. 15.
    Quan CP, Berneman A, Pires R, Avrameas S, Bouvet JP. Natural polyreactive secretory immunoglobulin A autoantibodies as a possible barrier to infection in humans. Infect Immun. 1997;65(10):3997–4004.PubMedGoogle Scholar
  16. 16.
    Vassilev TL, Veleva KV. Natural polyreactive IgA and IgM autoantibodies in human colostrum. Scand J Immunol. 1996;44(5):535–9.PubMedCrossRefGoogle Scholar
  17. 17.
    Mirilas P, Fesel C, Guilbert B, Beratis NG, Avrameas S. Natural antibodies in childhood: development, individual stability, and injury effect indicate a contribution to immune memory. J Clin Immunol. 1999;19(2):109–15.PubMedCrossRefGoogle Scholar
  18. 18.
    Coutinho A, Kazatchkine MD, Avrameas S. Natural autoantibodies. Curr Opin Immunol. 1995;7(6):812–8.PubMedCrossRefGoogle Scholar
  19. 19.
    Matheson MC, Allen KJ, Tang ML. Understanding the evidence for and against the role of breastfeeding in allergy prevention. Clin Exp Allergy. 2012. doi: 10.1111/j.1365-2222.2011.03925.x.
  20. 20.
    Kverka M, Burianova J, Lodinova-Zadnikova R, Kocourkova I, Cinova J, Tuckova L, et al. Cytokine profiling in human colostrum and milk by protein array. Clin Chem. 2007;53(5):955–62. doi: 10.1373/clinchem.2006.077107.PubMedCrossRefGoogle Scholar
  21. 21.
    Ferreira MU, Katzin AM. The assessment of antibody affinity distribution by thiocyanate elution: a simple dose-response approach. J Immunol Methods. 1995;187(2):297–305.PubMedCrossRefGoogle Scholar
  22. 22.
    Tuaillon E, Valea D, Becquart P, Al Tabaa Y, Meda N, Bollore K, et al. Human milk-derived B cells: a highly activated switched memory cell population primed to secrete antibodies. J Immunol. 2009;182(11):7155–62. doi: 10.4049/jimmunol.0803107.PubMedCrossRefGoogle Scholar
  23. 23.
    Mestecky J, Moro I, Kerr MA, Woof JM. Mucosal immunoglobulins. In: Mestecky J, Lamm ME, McGhee JR, Bienenstock J, Mayer L, Strober W, editors. Mucosal immunology. 3rd ed. Burlington: Academic Press; 2005. p. 153–81.CrossRefGoogle Scholar
  24. 24.
    Ladjeva I, Peterman JH, Mestecky J. IgA subclasses of human colostral antibodies specific for microbial and food antigens. Clin Exp Immunol. 1989;78(1):85–90.PubMedGoogle Scholar
  25. 25.
    Merbl Y, Zucker-Toledano M, Quintana FJ, Cohen IR. Newborn humans manifest autoantibodies to defined self molecules detected by antigen microarray informatics. J Clin Invest. 2007;117(3):712–8. doi: 10.1172/JCI29943.PubMedCrossRefGoogle Scholar
  26. 26.
    Cripps AW, Gleeson M. Ontogeny of mucosal immunity and aging. In: Mestecky J, Lamm ME, McGhee JR, Bienenstock J, Mayer L, Strober W, editors. Mucosal immunology. 3rd ed. Burlington: Academic Press; 2005. p. 305–21.CrossRefGoogle Scholar
  27. 27.
    Jacob CM, Pastorino AC, Fahl K, Carneiro-Sampaio M, Monteiro RC. Autoimmunity in IgA deficiency: revisiting the role of IgA as a silent housekeeper. J Clin Immunol. 2008;28 Suppl 1:S56–61. doi: 10.1007/s10875-007-9163-2.PubMedCrossRefGoogle Scholar
  28. 28.
    Mathew GD, Qualtiere LF, Neel 3rd HB, Pearson GR. IgA antibody, antibody-dependent cellular cytotoxicity and prognosis in patients with nasopharyngeal carcinoma. Int J Cancer. 1981;27(2):175–80.PubMedCrossRefGoogle Scholar
  29. 29.
    Lissauer DM, Piper KP, Moss PA, Kilby MD. Fetal microchimerism: the cellular and immunological legacy of pregnancy. Expert Rev Mol Med. 2009;11:e33. doi: 10.1017/S1462399409001264.PubMedCrossRefGoogle Scholar
  30. 30.
    Cukrowska B, Sinkora J, Rehakova Z, Sinkora M, Splichal I, Tuckova L, et al. Isotype and antibody specificity of spontaneously formed immunoglobulins in pig fetuses and germ-free piglets: production by CD5- B cells. Immunology. 1996;88(4):611–7.PubMedCrossRefGoogle Scholar
  31. 31.
    Kit YY, Starykovych MA, Richter VA, Stoika RS. Detection and characterization of IgG-and sIgA-abzymes capable of hydrolyzing histone H1. Biochemistry (Mosc). 2008;73(8):950–6. doi: 10.1134/S0006297908080142.CrossRefGoogle Scholar
  32. 32.
    Nevinsky GA, Buneva VN. Natural catalytic antibodies in norm, autoimmune, viral, and bacterial diseases. ScientificWorldJournal. 2010;10:1203–33. doi: 10.1100/Tsw.2010.98.PubMedCrossRefGoogle Scholar
  33. 33.
    Karataeva NA, Gorbunov D, Prokudin IV, Buneva VN, Kulminskaya AA, Neustroev KN, et al. Human milk antibodies with polysaccharide kinase activity. Immunol Lett. 2006;103(1):58–67. doi: 10.1016/j.imlet.2005.10.009.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Jaroslava Pribylova
    • 1
  • Klara Krausova
    • 1
  • Ingrid Kocourkova
    • 2
  • Pavel Rossmann
    • 1
  • Klara Klimesova
    • 1
  • Miloslav Kverka
    • 1
  • Helena Tlaskalova-Hogenova
    • 1
  1. 1.Institute of MicrobiologyAcademy of Sciences of the Czech RepublicPrague 4-KrčCzech Republic
  2. 2.Paediatric DepartmentInstitute for the Care of Mother and ChildPragueCzech Republic

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