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Journal of Clinical Immunology

, Volume 32, Issue 6, pp 1372–1380 | Cite as

Colostrum of Healthy Mothers Contains Broad Spectrum of Secretory IgA Autoantibodies

  • Jaroslava Pribylova
  • Klara Krausova
  • Ingrid Kocourkova
  • Pavel Rossmann
  • Klara Klimesova
  • Miloslav Kverka
  • Helena Tlaskalova-Hogenova
Article

Abstract

Purpose

Human colostrum and milk provide a newborn with immunomodulatory components, ensuring protection and proper development of the immune system. Secretory IgA antibodies in colostrum represent the first line of defence against harmful substances, but their potential spectra of reactivity with autoantigens remains unclear. Here, we characterised the repertoire of natural sectretory IgA autoantibodies in colostrum of healthy mothers.

Methods

The human colostrum samples from 39 healthy mothers were analyzed for autoantibodies by indirect immunofluorescence, dot blots, immunoblots and ELISA.

Results

We found that there is high diversity in reactivities of colostral IgA antibodies to autoantigens among individual samples. Using tissue sections and biochips commonly used for autoimmunity testing, we found that most samples reacted with monkey ovary (79.3 %), monkey pancreatic tissue (78.6 %), human HEp-2 cells (69 %) and monkey adrenal gland (69.0 %), fewer samples reacted with monkey liver tissue (47.2 %), rat stomach (42.9 %), monkey testicular tissue (41.4 %), monkey salivary gland (39.3 %), rat kidney (32.1 %) and monkey cerebellar tissue (17.9 %). At the protein level, we detected reactivity of IgA with 21 out of 25 (auto) antigens. The majority of the samples reacted with the pyruvate dehydrogenase complex, E3 ubiquitin ligase, cytosolic liver antigen, promyelocytic leukemia protein and nuclear pore glycoprotein-210. Using ELISA, we found reactivity of colostral IgA antibodies against examined extractable nuclear antigens, double stranded DNA, phospholipids and neutrophil cytoplasm.

Conclusions

The broad spectrum of polyreactive natural autoantibodies present in human colostrum may contribute to proper development of mucosal immune system of the breastfed infant.

Keywords

Autoantibodies mucosal immunity immunoglobulins immunohistochemistry/immunohistology anti-DNA antibodies 

Abbreviations

SIgA

secretory IgA

cIgA

colostral IgA

PBS

phosphate-buffered saline

NaSCN

sodium thiocyanate

Notes

Acknowledgments

This work was supported by grants: IAA500200910 (Grant Agency of the Academy of Sciences of the Czech Republic), P304/11/1252 (Czech Science Foundation), and RVO: 61388971 (Institutional Research Concept).

Supplementary material

10875_2012_9733_MOESM1_ESM.pdf (4.3 mb)
ESM 1 (PDF 4356 kb)

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Jaroslava Pribylova
    • 1
  • Klara Krausova
    • 1
  • Ingrid Kocourkova
    • 2
  • Pavel Rossmann
    • 1
  • Klara Klimesova
    • 1
  • Miloslav Kverka
    • 1
  • Helena Tlaskalova-Hogenova
    • 1
  1. 1.Institute of MicrobiologyAcademy of Sciences of the Czech RepublicPrague 4-KrčCzech Republic
  2. 2.Paediatric DepartmentInstitute for the Care of Mother and ChildPragueCzech Republic

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