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Journal of Clinical Immunology

, Volume 30, Issue 1, pp 68–73 | Cite as

Association of Interferon-Gamma Gene Polymorphisms in Taiwanese Children with Biliary Atresia

  • Hung-Chang Lee
  • Tzu-Yang Chang
  • Chun-Yan Yeung
  • Wai-Tao Chan
  • Chuen-Bin Jiang
  • Wei-Fang Chen
  • Hui-Wen Chan
  • Hsin-Fu Liu
  • Marie Lin
  • Yann-Jinn Lee
Article

Abstract

Background

Biliary atresia (BA) is a devastating neonatal hepatobiliary disease characterized by bile duct inflammation and fibrosis. The pathogenesis remains unclear, but immunologically mediated injury to bile ducts following an infectious insult is likely to play a critical role. Interferon-gamma (IFN-γ) is a key cytokine that affects immune-mediated inflammatory responses.

Objective

This study aims to investigate whether polymorphisms of the IFN-γ (IFNG) gene were associated with susceptibility to BA.

Methods

The IFNG −1615 C/T, −183 G/T, +874 A/T, and +2197 A/G polymorphisms were genotyped using the TaqMan assay, and CA repeat microsatellite was analyzed using capillary electrophoresis in 50 children with BA and 788 ethnically matched healthy controls.

Results

The distribution of genotype, allele, and haplotype frequencies of these IFNG gene variants did not differ significantly between children with BA and controls.

Conclusion

Polymorphisms of the IFNG gene do not appear to play a major role in the genetic predisposition to BA in Taiwanese children.

Keywords

Biliary atresia interferon-gamma inflammation polymorphism 

Notes

Acknowledgements

This research was supported by grants MMH-9780 and MMH E-97007 from Mackay Memorial Hospital, Taiwan.

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Hung-Chang Lee
    • 1
    • 3
  • Tzu-Yang Chang
    • 2
  • Chun-Yan Yeung
    • 1
    • 4
  • Wai-Tao Chan
    • 1
  • Chuen-Bin Jiang
    • 1
  • Wei-Fang Chen
    • 2
  • Hui-Wen Chan
    • 2
  • Hsin-Fu Liu
    • 2
  • Marie Lin
    • 2
  • Yann-Jinn Lee
    • 1
    • 2
    • 3
    • 5
  1. 1.Departments of PediatricsMackay Memorial HospitalTaipeiTaiwan
  2. 2.Medical ResearchMackay Memorial HospitalTaipeiTaiwan
  3. 3.Department of PediatricsTaipei Medical UniversityTaipeiTaiwan
  4. 4.Mackay MedicineNursing and Management CollegeTaipeiTaiwan
  5. 5.Departments of Pediatrics and Medical ResearchMackay Memorial HospitalTamshuiTaiwan

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