Association of Interferon-Gamma Gene Polymorphisms in Taiwanese Children with Biliary Atresia
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Biliary atresia (BA) is a devastating neonatal hepatobiliary disease characterized by bile duct inflammation and fibrosis. The pathogenesis remains unclear, but immunologically mediated injury to bile ducts following an infectious insult is likely to play a critical role. Interferon-gamma (IFN-γ) is a key cytokine that affects immune-mediated inflammatory responses.
This study aims to investigate whether polymorphisms of the IFN-γ (IFNG) gene were associated with susceptibility to BA.
The IFNG −1615 C/T, −183 G/T, +874 A/T, and +2197 A/G polymorphisms were genotyped using the TaqMan assay, and CA repeat microsatellite was analyzed using capillary electrophoresis in 50 children with BA and 788 ethnically matched healthy controls.
The distribution of genotype, allele, and haplotype frequencies of these IFNG gene variants did not differ significantly between children with BA and controls.
Polymorphisms of the IFNG gene do not appear to play a major role in the genetic predisposition to BA in Taiwanese children.
KeywordsBiliary atresia interferon-gamma inflammation polymorphism
This research was supported by grants MMH-9780 and MMH E-97007 from Mackay Memorial Hospital, Taiwan.
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