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Journal of Clinical Immunology

, Volume 28, Issue 5, pp 571–580 | Cite as

Histamine Release and Inflammatory Cell Infiltration in Airway Mucosa in Methylene Diphenyl Diisocyanate (MDI)-Induced Occupational Asthma

  • Gyu-Young Hur
  • Seung-Soo Sheen
  • Young-Mi Kang
  • Dong-Hee Koh
  • Han-Jung Park
  • Young-Min Ye
  • Hyun-ee Yim
  • Kyoo-Sang Kim
  • Hae-Sim Park
Article

Abstract

Introduction

Although methylene diphenyl diisocyanate (MDI) is widely used in industries, there have been few studies of the pathogenic mechanisms of MDI-induced occupational asthma (MDI-OA).

Methods

We performed immunohistochemical analyses, measured inflammatory mediators and cytokines, and quantified histamine release (HR) from peripheral basophils in MDI-OA patients. Thirteen MDI-exposed workers (five MDI-OA, two MDI-induced esoinophilic bronchitis, and six asymptomatic exposed controls, AEC) were enrolled.

Results and Discussion

Immunochemical analyses indicated significantly increased anti-eosinophilic cationic protein-stained cells in MDI-OA patients as compared with controls (P < 0.05). Sputum eosinophil cationic protein levels were increased after MDI-specific inhalation challenge test in MDI-OA/EB patients (P < 0.02). Sputum eosinophil counts were highly correlated with IL-8 and MMP-9 levels (P < 0.05 and P < 0.01, respectively). Basophil HR was significantly increased in MDI-OA patients after stimulations with anti-IgG4 and MDI–human serum albumin conjugates (both P < 0.05). Eosinophil activation is a major feature of airway inflammation in MDI-OA patients. Increased HR by MDI may contribute to the pathogenic mechanisms of MDI-OA.

Keywords

Eosinophil mast cell MDI occupational asthma 

Abbreviations

AA

allergic asthma

AEC

asymptomatic exposed controls

ECP

eosinophil cationic protein

HSA

human serum albumin

HR

histamine release

MDI

methylene diphenyl diisocyanate

MDI-EB

methylene diphenyl diisocyanate-induced occupational eosinophilic bronchitis

MDI-OA

methylene diphenyl diisocyanate-induced occupational asthma

MDI-OA/EB

methylene diphenyl diisocyanate-induced occupational asthma or eosinophilic bronchitis

MMP-9

matrix metalloproteinase-9

MPO

myeloperoxidase

TDI

toluene diisocyanate

TDI-OA

toluene diisocyanate-induced occupational asthma

TIMP1

tissue inhibitor of metalloproteinase-1

VEGF

vascular endothelial growth factor

WRRS

work-related respiratory symptoms

Notes

Acknowledgment

We would like to thank Dr. Adam Wisnewski of Yale University for providing the MDI–HSA conjugates used in this study. This study was supported by a grant from the Korean Health 21 R&D Project of the Ministry of Health and Welfare, Republic of Korea (A050571).

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Gyu-Young Hur
    • 1
  • Seung-Soo Sheen
    • 2
  • Young-Mi Kang
    • 1
  • Dong-Hee Koh
    • 4
  • Han-Jung Park
    • 1
  • Young-Min Ye
    • 1
  • Hyun-ee Yim
    • 3
  • Kyoo-Sang Kim
    • 4
  • Hae-Sim Park
    • 1
  1. 1.Department of Allergy and RheumatologyAjou University School of MedicineYoungtong-guKorea
  2. 2.Department of Pulmonary and Critical Care MedicineAjou University School of MedicineSuwonKorea
  3. 3.Department of PathologyAjou University School of MedicineSuwonKorea
  4. 4.Occupational Safety and Health Research InstituteKorea Occupational Safety and Health AgencyIncheonKorea

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