Journal of Clinical Immunology

, Volume 25, Issue 1, pp 50–56 | Cite as

Long-Term Effects of IFN-γ, IL-10, and TGF-β-Modulated Dendritic Cells on Immune Response in Lewis Rats



Increasing data have shown that IFN-γ, IL-10, and TGF-β-modulated dendritic cells (DC) provide a promising strategy in treatment of experimental allergic encephalomyelitis and experimental autoimmune myasthenia gravis through different manner. To explore the immune response status after long-term application of these cytokine-modulated-DC, Lewis rats were injected subcutaneously into naive DC and IFN-γ, IL-10, and TGF-β-modulated DC (i.e., IFN-γ-DC, IL-10-DC, and TGF-β-DC) at does of 1 × 106 cells/rat every month for continuous 18 months, respectively. No rats suffered from decreased vigor and activity as well as cachectic condition during 18-month observation, and no rats had body-weight loss after 18-month treatment. Exploratory laparectomy did not find any tumor in all rats. IL-10-DC and TGF-β-DC resulted in lower nonspecific (Con A-induced) and antigen specific (ovalbumin-stimulated) spleen mononuclear cells proliferation, accompanied by lower levels of IFN-γ, IL-10, and TNF-α. On the contrary, IFN-γ-DC did not suppress cell proliferation and IFN-γ and IL-10 production except only slightly decreased TNF-α levels. These results suggest that IFN-γ-DC seems to be a more ideal candidate in the treatment of autoimmune diseases without suppressing immune response.


Autoimmune disease cytokine dendritic cell proliferation 


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Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  1. 1.Division of Neuroimmunology NeurotecKarolinska InstituteStockholmSweden
  2. 2.Institute of NeurologyFudan UniversityShanghaiChina
  3. 3.Division of NeurologyShandong Qianfoshan HospitalShandongChina
  4. 4.Division of Neuroimmunology NeurotecKarolinska InstituteStockholmSweden
  5. 5.Division of NeurologyShandong Qianfoshan HospitalShandongChina

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